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2uun

From Proteopedia

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[[Image:2uun.png|left|200px]]
 
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==Crystal structure of C-phycocyanin from Phormidium, Lyngbya spp. (Marine) and Spirulina sp. (Fresh water) shows two different ways of energy transfer between two hexamers.==
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The line below this paragraph, containing "STRUCTURE_2uun", creates the "Structure Box" on the page.
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<StructureSection load='2uun' size='340' side='right'caption='[[2uun]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2uun]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptolyngbya_sp. Leptolyngbya sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UUN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene>, <scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gh0|1gh0]], [[2uul|2uul]], [[2uum|2uum]]</div></td></tr>
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{{STRUCTURE_2uun| PDB=2uun | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uun OCA], [https://pdbe.org/2uun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uun RCSB], [https://www.ebi.ac.uk/pdbsum/2uun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uun ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uu/2uun_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uun ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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C-phycocyanins from three cyanobacterial cultures of freshwater and marine habitat, Spirulina, Phormidium and Lyngbya spp., were purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Blue-coloured crystals in different crystal forms, monoclinic and hexagonal, were obtained for the three species. The crystals took 1-12 weeks to grow to full size using polyethylene glycols of different molecular weights as precipitants. The amino-acid sequences of these proteins show high similarity to other known C-phycocyanins from related organisms; however, the C-phycocyanins reported here showed different biochemical and biophysical properties, i.e. molecular weight, stability etc. The X-ray diffraction data were collected at resolutions of 3.0 A for the monoclinic and 3.2 and 3.6 A for the hexagonal forms. The unit-cell parameters corresponding to the monoclinic space group P2(1) are a = 107.33, b = 115.64, c = 183.26 A, beta = 90.03 degrees for Spirulina sp. C-phycocyanin and are similar for crystals of Phormidium and Lyngbya spp. C-phycocyanins. Crystals belonging to the hexagonal space group P6(3), with unit-cell parameters a = b = 154.97, c = 40.35 A and a = b = 151.96, c = 39.06 A, were also obtained for the C-phycocyanins from Spirulina and Lyngbya spp., respectively. The estimated solvent content is around 50% for the monoclinic crystals of all three species assuming the presence of two hexamers per asymmetric unit. The solvent content is 66.5 and 64.1% for the hexagonal crystals of C-phycocyanin from Spirulina and Lyngbya spp. assuming the presence of one alphabeta monomer per asymmetric unit.
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===CRYSTAL STRUCTURE OF C-PHYCOCYANIN FROM PHORMIDIUM, LYNGBYA SPP. (MARINE) AND SPIRULINA SP. (FRESH WATER) SHOWS TWO DIFFERENT WAYS OF ENERGY TRANSFER BETWEEN TWO HEXAMERS.===
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X-ray crystallographic studies on C-phycocyanins from cyanobacteria from different habitats: marine and freshwater.,Satyanarayana L, Suresh CG, Patel A, Mishra S, Ghosh PK Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Sep 1;61(Pt, 9):844-7. Epub 2005 Aug 31. PMID:16511175<ref>PMID:16511175</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2uun" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16511175}}, adds the Publication Abstract to the page
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*[[Phycocyanin|Phycocyanin]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16511175 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16511175}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[2uun]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Leptolyngbya_sp. Leptolyngbya sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUN OCA].
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[[Category: Leptolyngbya sp]]
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[[Category: Ghosh, P K]]
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==Reference==
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[[Category: Mishra, S]]
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<ref group="xtra">PMID:16511175</ref><ref group="xtra">PMID:15766866</ref><references group="xtra"/>
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[[Category: Patel, A]]
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[[Category: Leptolyngbya sp.]]
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[[Category: Satyanarayana, L]]
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[[Category: Ghosh, P K.]]
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[[Category: Suresh, C G]]
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[[Category: Mishra, S.]]
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[[Category: Patel, A.]]
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[[Category: Satyanarayana, L.]]
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[[Category: Suresh, C G.]]
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[[Category: C-phycocyanin]]
[[Category: C-phycocyanin]]
[[Category: Electron transport]]
[[Category: Electron transport]]

Current revision

Crystal structure of C-phycocyanin from Phormidium, Lyngbya spp. (Marine) and Spirulina sp. (Fresh water) shows two different ways of energy transfer between two hexamers.

PDB ID 2uun

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