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3ky9

From Proteopedia

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[[Image:3ky9.png|left|200px]]
 
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==Autoinhibited Vav1==
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The line below this paragraph, containing "STRUCTURE_3ky9", creates the "Structure Box" on the page.
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<StructureSection load='3ky9' size='340' side='right' caption='[[3ky9]], [[Resolution|resolution]] 2.73&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ky9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KY9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KY9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VAV, VAV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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{{STRUCTURE_3ky9| PDB=3ky9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ky9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ky9 OCA], [http://pdbe.org/3ky9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ky9 RCSB], [http://www.ebi.ac.uk/pdbsum/3ky9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ky9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/VAV_HUMAN VAV_HUMAN]] Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ky/3ky9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ky9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Vav proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases. They control processes including T cell activation, phagocytosis, and migration of normal and transformed cells. We report the structure and biophysical and cellular analyses of the five-domain autoinhibitory element of Vav1. The catalytic Dbl homology (DH) domain of Vav1 is controlled by two energetically coupled processes. The DH active site is directly, but weakly, inhibited by a helix from the adjacent Acidic domain. This core interaction is strengthened 10-fold by contacts of the calponin homology (CH) domain with the Acidic, pleckstrin homology, and DH domains. This construction enables efficient, stepwise relief of autoinhibition: initial phosphorylation events disrupt the modulatory CH contacts, facilitating phosphorylation of the inhibitory helix and consequent GEF activation. Our findings illustrate how the opposing requirements of strong suppression of activity and rapid kinetics of activation can be achieved in multidomain systems.
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===Autoinhibited Vav1===
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Structural and energetic mechanisms of cooperative autoinhibition and activation of Vav1.,Yu B, Martins IR, Li P, Amarasinghe GK, Umetani J, Fernandez-Zapico ME, Billadeau DD, Machius M, Tomchick DR, Rosen MK Cell. 2010 Jan 22;140(2):246-56. PMID:20141838<ref>PMID:20141838</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20141838}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3ky9" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20141838 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20141838}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Human]]
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[[3ky9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KY9 OCA].
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[[Category: Machius, M]]
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[[Category: Rosen, M K]]
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==Reference==
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[[Category: Tomchick, D R]]
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<ref group="xtra">PMID:20141838</ref><ref group="xtra">PMID:18488041</ref><ref group="xtra">PMID:16285729</ref><ref group="xtra">PMID:11007481</ref><references group="xtra"/>
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[[Category: Yu, B]]
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[[Category: Homo sapiens]]
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[[Category: Machius, M.]]
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[[Category: Rosen, M K.]]
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[[Category: Tomchick, D R.]]
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[[Category: Yu, B.]]
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[[Category: Apoptosis]]
[[Category: Apoptosis]]
[[Category: C1 domain]]
[[Category: C1 domain]]

Current revision

Autoinhibited Vav1

3ky9, resolution 2.73Å

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