3f4i

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[[Image:3f4i.png|left|200px]]
 
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==Crystal Structure of LeuT bound to L-selenomethionine and sodium==
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The line below this paragraph, containing "STRUCTURE_3f4i", creates the "Structure Box" on the page.
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<StructureSection load='3f4i' size='340' side='right'caption='[[3f4i]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3f4i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F4I FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_3f4i| PDB=3f4i | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f4i OCA], [https://pdbe.org/3f4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f4i RCSB], [https://www.ebi.ac.uk/pdbsum/3f4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f4i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O67854_AQUAE O67854_AQUAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f4/3f4i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f4i ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Secondary transporters are workhorses of cellular membranes, catalyzing the movement of small molecules and ions across the bilayer and coupling substrate passage to ion gradients. However, the conformational changes that accompany substrate transport, the mechanism by which a substrate moves through the transporter, and principles of competitive inhibition remain unclear. We used crystallographic and functional studies on the leucine transporter (LeuT), a model for neurotransmitter sodium symporters, to show that various amino acid substrates induce the same occluded conformational state and that a competitive inhibitor, tryptophan (Trp), traps LeuT in an open-to-out conformation. In the Trp complex, the extracellular gate residues arginine 30 and aspartic acid 404 define a second weak binding site for substrates or inhibitors as they permeate from the extracellular solution to the primary substrate site, which demonstrates how residues that participate in gating also mediate permeation.
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===Crystal Structure of LeuT bound to L-selenomethionine and sodium===
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A competitive inhibitor traps LeuT in an open-to-out conformation.,Singh SK, Piscitelli CL, Yamashita A, Gouaux E Science. 2008 Dec 12;322(5908):1655-61. PMID:19074341<ref>PMID:19074341</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3f4i" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19074341}}, adds the Publication Abstract to the page
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*[[Leucine transporter|Leucine transporter]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19074341 is the PubMed ID number.
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*[[Symporter 3D structures|Symporter 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_19074341}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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[[3f4i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F4I OCA].
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==Reference==
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<ref group="xtra">PMID:19074341</ref><ref group="xtra">PMID:17687333</ref><ref group="xtra">PMID:16041361</ref><references group="xtra"/>
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[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
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[[Category: Gouaux, E.]]
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[[Category: Large Structures]]
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[[Category: Piscitelli, C L.]]
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[[Category: Gouaux E]]
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[[Category: Singh, S K.]]
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[[Category: Piscitelli CL]]
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[[Category: Yamashita, A.]]
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[[Category: Singh SK]]
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[[Category: Membrane protein]]
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[[Category: Yamashita A]]
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[[Category: Neurotransmitter]]
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[[Category: Nss]]
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[[Category: Slc6]]
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[[Category: Sodium-couple]]
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[[Category: Symport]]
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[[Category: Transmembrane]]
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[[Category: Transport]]
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[[Category: Transport protein]]
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[[Category: Transporter]]
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Current revision

Crystal Structure of LeuT bound to L-selenomethionine and sodium

PDB ID 3f4i

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OCA

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