2pqj

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[[Image:2pqj.png|left|200px]]
 
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==Crystal structure of active ribosome inactivating protein from maize (b-32), complex with adenine==
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The line below this paragraph, containing "STRUCTURE_2pqj", creates the "Structure Box" on the page.
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<StructureSection load='2pqj' size='340' side='right'caption='[[2pqj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pqj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PQJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene></td></tr>
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{{STRUCTURE_2pqj| PDB=2pqj | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pqj OCA], [https://pdbe.org/2pqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pqj RCSB], [https://www.ebi.ac.uk/pdbsum/2pqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pqj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIP3_MAIZE RIP3_MAIZE] Possesses features of some constitutive defense agent. The coordinate Opaque-2-controlled synthesis of this protein and the major seed storage proteins (zeins) may provide the germinating seedling with both nutritional benefits and protection against pathogen invasion of the surrounding endosperm.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pq/2pqj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pqj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Maize ribosome-inactivating protein is classified as a class III or an atypical RNA N-glycosidase. It is synthesized as an inactive precursor with a 25-amino acid internal inactivation region, which is removed in the active form. As the first structural example of this class of proteins, crystals of the precursor and the active form were diffracted to 2.4 and 2.5 A, respectively. The two proteins are similar, with main chain root mean square deviation (RMSD) of 0.519. In the precursor, the inactivation region is found on the protein surface and consists of a flexible loop followed by a long alpha-helix. This region diminished both the interaction with ribosome and cytotoxicity, but not cellular uptake. Like bacterial ribosome-inactivating proteins, maize ribosome-inactivating protein does not have a back-up glutamate in the active site, which helps the protein to retain some activity if the catalytic glutamate is mutated. The structure reveals that the active site is too small to accommodate two glutamate residues. Our structure suggests that maize ribosome-inactivating protein may represent an intermediate product in the evolution of ribosome-inactivating proteins.
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===Crystal structure of active ribosome inactivating protein from maize (b-32), complex with adenine===
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Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site.,Mak AN, Wong YT, An YJ, Cha SS, Sze KH, Au SW, Wong KB, Shaw PC Nucleic Acids Res. 2007;35(18):6259-67. Epub 2007 Sep 13. PMID:17855394<ref>PMID:17855394</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2pqj" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17855394}}, adds the Publication Abstract to the page
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*[[Ribosome inactivating protein 3D structures|Ribosome inactivating protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17855394 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17855394}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[2pqj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PQJ OCA].
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==Reference==
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<ref group="xtra">PMID:17855394</ref><ref group="xtra">PMID:1744135</ref><references group="xtra"/>
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[[Category: Zea mays]]
[[Category: Zea mays]]
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[[Category: RRNA N-glycosylase]]
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[[Category: Au SWN]]
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[[Category: Au, S W.N.]]
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[[Category: Cha SS]]
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[[Category: Cha, S S.]]
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[[Category: Mak ANS]]
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[[Category: Mak, A N.S.]]
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[[Category: Shaw PC]]
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[[Category: Shaw, P C.]]
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[[Category: Wong KB]]
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[[Category: Wong, K B.]]
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[[Category: Young JA]]
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[[Category: Young, J A.]]
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[[Category: Adenine]]
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[[Category: Cytoplasm]]
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[[Category: Hydrolase]]
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[[Category: Maize]]
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[[Category: Mod]]
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[[Category: Plant defense]]
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[[Category: Protein synthesis inhibitor]]
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[[Category: Ribosome inactivating protein]]
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[[Category: Toxin]]
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Current revision

Crystal structure of active ribosome inactivating protein from maize (b-32), complex with adenine

PDB ID 2pqj

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