3n9h

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[[Image:3n9h.png|left|200px]]
 
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==Crystal Structural of mutant Y305A in the copper amine oxidase from hansenula polymorpha==
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The line below this paragraph, containing "STRUCTURE_3n9h", creates the "Structure Box" on the page.
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<StructureSection load='3n9h' size='340' side='right'caption='[[3n9h]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3n9h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Ogataea_angusta Ogataea angusta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N9H FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene></td></tr>
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{{STRUCTURE_3n9h| PDB=3n9h | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9h OCA], [https://pdbe.org/3n9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n9h RCSB], [https://www.ebi.ac.uk/pdbsum/3n9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n9h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMO_PICAN AMO_PICAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n9/3n9h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n9h ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The copper amine oxidases carry out two copper-dependent processes: production of their own redox-active cofactor (2,4,5-trihydroxyphenylalanine quinone, TPQ) and the subsequent oxidative deamination of substrate amines. Because the same active site pocket must facilitate both reactions, individual active site residues may serve multiple roles. We have examined the roles of a strictly conserved active site tyrosine Y305 in the copper amine oxidase from Hansenula polymorpha kinetically, spetroscopically (Dubois and Klinman (2006) Biochemistry 45, 3178), and, in the present work, structurally. While the Y305A enzyme is almost identical to the wild type, a novel, highly oxygenated species replaces TPQ in the Y305F active sites. This new structure not only provides the first direct detection of peroxy intermediates in cofactor biogenesis but also indicates the critical control of oxidation chemistry that can be conferred by a single active site residue.
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===Crystal Structural of mutant Y305A in the copper amine oxidase from hansenula polymorpha===
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Mutation at a strictly conserved, active site tyrosine in the copper amine oxidase leads to uncontrolled oxygenase activity.,Chen ZW, Datta S, Dubois JL, Klinman JP, Mathews FS Biochemistry. 2010 Aug 31;49(34):7393-402. PMID:20684524<ref>PMID:20684524</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3n9h" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20684524}}, adds the Publication Abstract to the page
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*[[Copper amine oxidase 3D structures|Copper amine oxidase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20684524 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20684524}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3n9h]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pichia_angusta Pichia angusta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9H OCA].
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[[Category: Ogataea angusta]]
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[[Category: Chen Z]]
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==Reference==
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[[Category: Datta S]]
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<ref group="xtra">PMID:20684524</ref><ref group="xtra">PMID:9551552</ref><references group="xtra"/>
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[[Category: DuBois JL]]
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[[Category: Pichia angusta]]
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[[Category: Klinman JP]]
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[[Category: Primary-amine oxidase]]
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[[Category: Mathews FS]]
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[[Category: Chen, Z.]]
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[[Category: Datta, S.]]
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[[Category: DuBois, J L.]]
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[[Category: Klinman, J P.]]
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[[Category: Mathews, F S.]]
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[[Category: Amine oxidase]]
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[[Category: Oxidoreductase]]
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[[Category: Quinoprotein]]
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Current revision

Crystal Structural of mutant Y305A in the copper amine oxidase from hansenula polymorpha

PDB ID 3n9h

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