2ebs

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[[Image:2ebs.png|left|200px]]
 
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==Crystal Structure Anaalysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH) D465N Mutant Complexed with a Xyloglucan Heptasaccharide==
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The line below this paragraph, containing "STRUCTURE_2ebs", creates the "Structure Box" on the page.
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<StructureSection load='2ebs' size='340' side='right'caption='[[2ebs]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ebs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geotrichum_sp._M128 Geotrichum sp. M128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EBS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr>
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{{STRUCTURE_2ebs| PDB=2ebs | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ebs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebs OCA], [https://pdbe.org/2ebs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ebs RCSB], [https://www.ebi.ac.uk/pdbsum/2ebs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ebs ProSAT], [https://www.topsan.org/Proteins/RSGI/2ebs TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CBHRE_GEOS1 CBHRE_GEOS1]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/2ebs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ebs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Oligoxyloglucan reducing end-specific cellobiohydrolase (OXG-RCBH) is a unique exo-beta-1,4-glucanase that belongs to glycoside hydrolase family 74. The enzyme recognizes the reducing end of xyloglucan oligosaccharides and releases two glucosyl residue segments from the reducing end of the main chain. Previously, we reported that OXG-RCBH consists of two seven-bladed beta-propeller domains. There is a large cleft between the two domains, and a unique loop encloses one side of the active site cleft. Here, we report the X-ray crystal structure of the OXG-RCBH-substrate complex determined to a resolution of 2.4 A. The substrate bound to the cleft, and its reducing end was arranged near the loop region that is believed to impart OXG-RCBH with its activity. We constructed a deletion mutant of the loop region and conducted a detailed analysis. A deletion mutant of the loop region showed endo-activity with altered substrate recognition. More specifically, cleavage occurred randomly instead of at specific sites, most likely due to the misalignment of the substrate within the subsite. We believe that the loop imparts unique substrate specificity with exo-mode hydrolysis in OXG-RCBH.
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===Crystal Structure Anaalysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH) D465N Mutant Complexed with a Xyloglucan Heptasaccharide===
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The structural basis for the exo-mode of action in GH74 oligoxyloglucan reducing end-specific cellobiohydrolase.,Yaoi K, Kondo H, Hiyoshi A, Noro N, Sugimoto H, Tsuda S, Mitsuishi Y, Miyazaki K J Mol Biol. 2007 Jun 29;370(1):53-62. Epub 2007 Apr 19. PMID:17498741<ref>PMID:17498741</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ebs" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17498741}}, adds the Publication Abstract to the page
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*[[Cellobiohydrolase 3D structures|Cellobiohydrolase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17498741 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17498741}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Geotrichum sp. M128]]
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[[2ebs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geotrichum_sp._m128 Geotrichum sp. m128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBS OCA].
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[[Category: Large Structures]]
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[[Category: Hiyoshi A]]
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==Reference==
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[[Category: Kondo H]]
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<ref group="xtra">PMID:17498741</ref><ref group="xtra">PMID:15242597</ref><references group="xtra"/>
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[[Category: Miyazaki K]]
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[[Category: Geotrichum sp. m128]]
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[[Category: Noro N]]
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[[Category: Oligoxyloglucan reducing-end-specific cellobiohydrolase]]
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[[Category: Sugimoto H]]
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[[Category: Hiyoshi, A.]]
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[[Category: Yaoi K]]
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[[Category: Kondo, H.]]
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[[Category: Miyazaki, K.]]
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[[Category: Noro, N.]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Sugimoto, H.]]
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[[Category: Yaoi, K.]]
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[[Category: Beta-propeller]]
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[[Category: Hydrolase]]
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[[Category: National project on protein structural and functional analyse]]
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[[Category: Nppsfa]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: Rsgi]]
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[[Category: Structural genomic]]
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Current revision

Crystal Structure Anaalysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH) D465N Mutant Complexed with a Xyloglucan Heptasaccharide

PDB ID 2ebs

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