2a3g

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (05:04, 17 October 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2a3g.png|left|200px]]
 
-
<!--
+
==The structure of T6 bovine insulin==
-
The line below this paragraph, containing "STRUCTURE_2a3g", creates the "Structure Box" on the page.
+
<StructureSection load='2a3g' size='340' side='right'caption='[[2a3g]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2a3g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A3G FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_2a3g| PDB=2a3g | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3g OCA], [https://pdbe.org/2a3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a3g RCSB], [https://www.ebi.ac.uk/pdbsum/2a3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a3g ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/INS_BOVIN INS_BOVIN] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/2a3g_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a3g ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Porcine insulin differs in sequence from bovine insulin at residues A8 (Thr in porcine--&gt;Ala in bovine) and A10 (Ile in porcine--&gt;Val in bovine). The structure of T6 hexameric bovine insulin has been determined to 2.25 A resolution at room temperature and refined to a residual of 0.162. The structure of the independent dimer is nearly identical to the T6 porcine insulin dimer: the mean displacement of all backbone atoms is 0.16 A, with the largest displacements occurring at AlaB30. Each of two independent zinc ions is octahedrally coordinated by three HisB10 side chains and three water molecules. As has been observed in both human and porcine insulin, the GluB13 side chains are directed towards the center of the hexamer, where a short contact of 2.57 A occurs between two independent carboxyl O atoms, again suggesting the presence of a centered hydrogen bond. No significant displacements of backbone atoms or changes in conformation are observed at A8 or A10. Since there are no interhexamer hydrogen-bonded contacts involving A8 in either porcine or bovine insulin, the change in the identity of this residue appears to have little or no effect upon the packing of the hexamers in the unit cell. In contrast, the side chains of the three A10 residues in one trimer make van der Waals contacts with the A10 side chains in a translationally related hexamer. As a consequence of the loss of the C(delta1) atom from the isoleucine residue in porcine insulin to produce valine in bovine insulin, there is a 0.36 A decrease in the distance between independent pairs of C(beta) atoms and a 0.24 A decrease in the c dimension of the unit cell. Thus, the net effect of the change in sequence at A10 is to strengthen the stabilizing hydrophobic interactions between hexamers.
-
===The structure of T6 bovine insulin===
+
The structure of T6 bovine insulin.,Smith GD, Pangborn WA, Blessing RH Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1476-82. Epub 2005, Oct 19. PMID:16239724<ref>PMID:16239724</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2a3g" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_16239724}}, adds the Publication Abstract to the page
+
*[[Insulin 3D Structures|Insulin 3D Structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 16239724 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_16239724}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
[[2a3g]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3G OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:16239724</ref><ref group="xtra">PMID:2905485</ref><ref group="xtra">PMID:12595704</ref><ref group="xtra">PMID:12876340</ref><references group="xtra"/>
+
[[Category: Bos taurus]]
[[Category: Bos taurus]]
-
[[Category: Blessing, R H.]]
+
[[Category: Large Structures]]
-
[[Category: Pangborn, W A.]]
+
[[Category: Blessing RH]]
-
[[Category: Smith, G D.]]
+
[[Category: Pangborn WA]]
-
[[Category: Carbohydrate metabolism]]
+
[[Category: Smith GD]]
-
[[Category: Hormone]]
+
-
[[Category: Hormone/growth factor complex]]
+
-
[[Category: Insulin family]]
+
-
[[Category: Signal]]
+

Current revision

The structure of T6 bovine insulin

PDB ID 2a3g

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2a3g"
Personal tools