3fpw

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[[Image:3fpw.png|left|200px]]
 
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==Crystal Structure of HbpS with bound iron==
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The line below this paragraph, containing "STRUCTURE_3fpw", creates the "Structure Box" on the page.
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<StructureSection load='3fpw' size='340' side='right'caption='[[3fpw]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fpw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_reticuli Streptomyces reticuli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FPW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_3fpw| PDB=3fpw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpw OCA], [https://pdbe.org/3fpw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fpw RCSB], [https://www.ebi.ac.uk/pdbsum/3fpw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fpw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9RIM2_STRRE Q9RIM2_STRRE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/3fpw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fpw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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HbpS, a novel protein of previously unknown function from Streptomyces reticuli, is up-regulated in response to haemin- and peroxide-based oxidative stress and interacts with the SenS/SenR two-component signal transduction system. In this study, we report the high-resolution crystal structures (2.2 and 1.6 A) of octomeric HbpS crystallized in the presence and in the absence of haem and demonstrate that iron binds to surface-exposed lysine residues of an octomeric assembly. Based on an analysis of the crystal structures, we propose that the iron atom originates from the haem group and report subsequent biochemical experiments that demonstrate that HbpS possesses haem-degrading activity in vitro. Further examination of the crystal structures has identified amino acids that are essential for assembly of the octomer. The role of these residues is confirmed by biophysical experiments. Additionally, we show that while the octomeric assembly state of HbpS is not essential for haem-degrading activity, the assembly of HbpS is required for its interaction with the cognate sensor kinase, SenS. Homologs of HbpS and SenS/SenR have been identified in a number of medically and ecologically relevant bacterial species (including Vibrio cholerae, Klebsiella pneumoniae, Corynebacterium diphtheriae, Arthrobacter aurescens and Pseudomonas putida), suggesting the existence of a previously undescribed bacterial oxidative stress-response pathway common to Gram-negative and Gram-positive bacteria. Thus, the data presented provide the first insight into the function of a novel protein family and an example of an iron-mediated interaction between an accessory protein and its cognate two-component sensor kinase.
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===Crystal Structure of HbpS with bound iron===
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The oligomeric assembly of the novel haem-degrading protein HbpS is essential for interaction with its cognate two-component sensor kinase.,Lucana DO, Bogel G, Zou P, Groves MR J Mol Biol. 2009 Mar 6;386(4):1108-22. PMID:19244623<ref>PMID:19244623</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19244623}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3fpw" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19244623 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19244623}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3fpw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_reticuli Streptomyces reticuli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPW OCA].
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==Reference==
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<ref group="xtra">PMID:19244623</ref><ref group="xtra">PMID:18453708</ref><references group="xtra"/>
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[[Category: Streptomyces reticuli]]
[[Category: Streptomyces reticuli]]
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[[Category: Bogel, G.]]
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[[Category: Bogel G]]
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[[Category: Groves, M R.]]
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[[Category: Groves MR]]
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[[Category: Lucana, D Ortiz de Orue.]]
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[[Category: Ortiz de Orue Lucana D]]
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[[Category: Zou, P.]]
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[[Category: Zou P]]
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[[Category: Haem binding]]
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[[Category: Heme binding protein]]
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Current revision

Crystal Structure of HbpS with bound iron

PDB ID 3fpw

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