This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2ea1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2ea1" [edit=sysop:move=sysop])
Current revision (17:22, 15 December 2021) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2ea1.png|left|200px]]
 
-
<!--
+
==Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE==
-
The line below this paragraph, containing "STRUCTURE_2ea1", creates the "Structure Box" on the page.
+
<StructureSection load='2ea1' size='340' side='right'caption='[[2ea1]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GPG:GUANYLYL-2,5-PHOSPHOGUANOSINE'>GPG</scene></td></tr>
-
-->
+
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Enterobacter_ribonuclease Enterobacter ribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.6 3.1.27.6] </span></td></tr>
-
{{STRUCTURE_2ea1| PDB=2ea1 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [https://pdbe.org/2ea1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ea1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ea1 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[[https://www.uniprot.org/uniprot/RNI_ECOLI RNI_ECOLI]] One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/2ea1_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ea1 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
We have constructed a strain that overproduces ribonuclease I of Escherichia coli and we have purified large quantities of the enzyme. Data from fluorescence, CD, and DSC measurements showed that it was a very stable protein. The conformation energy determined from urea and guanidine hydrochloride denaturation experiments was 11.5 kcal mol(-1) at pH 7.5. Thermal denaturation studies indicated that it had a T(m) of 64 degrees C at pH 4.0. RNase I belongs to the RNase T2/S-RNase group of endoribonucleases, but near the amino terminus it has an unusually long hydrophilic segment. Part of this was removed in the deletion construct, RNase I Delta(26-38). We have obtained crystals of both RNase I and of an enzyme-G2'p5'G complex in the P2(1) space group and oligonucleotide complexes with both wild type and mutant enzymes. The current study lays the groundwork for extensive investigation into the structure, function, and physical properties of this widely distributed group of ribonucleases.
-
===Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE===
+
Overexpression, biophysical characterization, and crystallization of ribonuclease I from Escherichia coli, a broad-specificity enzyme in the RNase T2 family.,Padmanabhan S, Zhou K, Chu CY, Lim RW, Lim LW Arch Biochem Biophys. 2001 Jun 1;390(1):42-50. PMID:11368513<ref>PMID:11368513</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2ea1" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_11368513}}, adds the Publication Abstract to the page
+
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 11368513 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_11368513}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Bacillus coli migula 1895]]
-
[[2ea1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:11368513</ref><ref group="xtra">PMID:2541256</ref><references group="xtra"/>
+
[[Category: Enterobacter ribonuclease]]
[[Category: Enterobacter ribonuclease]]
-
[[Category: Escherichia coli]]
+
[[Category: Large Structures]]
-
[[Category: Lim, L W.]]
+
[[Category: Lim, L W]]
-
[[Category: Lim, R W.]]
+
[[Category: Lim, R W]]
-
[[Category: Padmanabhan, S.]]
+
[[Category: Padmanabhan, S]]
-
[[Category: Pan, J.]]
+
[[Category: Pan, J]]
-
[[Category: Zhou, K.]]
+
[[Category: Zhou, K]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Protein-gpg complex]]
[[Category: Protein-gpg complex]]

Current revision

Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE

PDB ID 2ea1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ea1"
Personal tools