2zhx

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[[Image:2zhx.png|left|200px]]
 
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==Crystal structure of Uracil-DNA Glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor==
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The line below this paragraph, containing "STRUCTURE_2zhx", creates the "Structure Box" on the page.
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<StructureSection load='2zhx' size='340' side='right'caption='[[2zhx]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zhx]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_PBS2 Bacillus phage PBS2] and [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZHX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zhx OCA], [https://pdbe.org/2zhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zhx RCSB], [https://www.ebi.ac.uk/pdbsum/2zhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zhx ProSAT]</span></td></tr>
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{{STRUCTURE_2zhx| PDB=2zhx | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UNG_MYCTU UNG_MYCTU] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zh/2zhx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zhx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Uracil-DNA glycosylase (UNG), a repair enzyme involved in the excision of uracil from DNA, from mycobacteria differs from UNGs from other sources, particularly in the sequence in the catalytically important loops. The structure of the enzyme from Mycobacterium tuberculosis (MtUng) in complex with a proteinaceous inhibitor (Ugi) has been determined by X-ray analysis of a crystal containing seven crystallographically independent copies of the complex. This structure provides the first geometric characterization of a mycobacterial UNG. A comparison of the structure with those of other UNG proteins of known structure shows that a central core region of the molecule is relatively invariant in structure and sequence, while the N- and C-terminal tails exhibit high variability. The tails are probably important in folding and stability. The mycobacterial enzyme exhibits differences in UNG-Ugi interactions compared with those involving UNG from other sources. The MtUng-DNA complex modelled on the basis of the known structure of the complex involving the human enzyme indicates a domain closure in the enzyme when binding to DNA. The binding involves a larger burial of surface area than is observed in binding by human UNG. The DNA-binding site of MtUng is characterized by the presence of a higher proportion of arginyl residues than is found in the binding site of any other UNG of known structure. In addition to the electrostatic effects produced by the arginyl residues, the hydrogen bonds in which they are involved compensate for the loss of some interactions arising from changes in amino-acid residues, particularly in the catalytic loops. The results arising from the present investigation represent unique features of the structure and interaction of mycobacterial Ungs.
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===Crystal structure of Uracil-DNA Glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor===
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Unique features of the structure and interactions of mycobacterial uracil-DNA glycosylase: structure of a complex of the Mycobacterium tuberculosis enzyme in comparison with those from other sources.,Kaushal PS, Talawar RK, Krishna PD, Varshney U, Vijayan M Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):551-60. Epub 2008, Apr 19. PMID:18453691<ref>PMID:18453691</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2zhx" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18453691}}, adds the Publication Abstract to the page
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*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18453691 is the PubMed ID number.
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*[[Uracil glycosylase inhibitor|Uracil glycosylase inhibitor]]
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== References ==
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{{ABSTRACT_PUBMED_18453691}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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[[2zhx]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_pbs2 Bacillus phage pbs2] and [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZHX OCA].
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[[Category: Bacillus phage PBS2]]
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[[Category: Large Structures]]
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==Reference==
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[[Category: Mycobacterium tuberculosis H37Rv]]
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<ref group="xtra">PMID:18453691</ref><ref group="xtra">PMID:12136137</ref><ref group="xtra">PMID:9776748</ref><references group="xtra"/>
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[[Category: Kaushal PS]]
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[[Category: Bacillus phage pbs2]]
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[[Category: Krishna PDV]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Talawar RK]]
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[[Category: Uridine nucleosidase]]
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[[Category: Varshney U]]
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[[Category: Kaushal, P S.]]
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[[Category: Vijayan M]]
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[[Category: Krishna, P D.V.]]
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[[Category: Talawar, R K.]]
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[[Category: Varshney, U.]]
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[[Category: Vijayan, M.]]
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[[Category: Cytoplasm]]
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[[Category: Dna damage]]
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[[Category: Dna repair]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Hydrolase/hydrolase inhibitor complex]]
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[[Category: Ung-dna interaction]]
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[[Category: Ung-ugi complex]]
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Current revision

Crystal structure of Uracil-DNA Glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor

PDB ID 2zhx

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