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3hbr

From Proteopedia

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[[Image:3hbr.png|left|200px]]
 
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==Crystal structure of OXA-48 beta-lactamase==
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The line below this paragraph, containing "STRUCTURE_3hbr", creates the "Structure Box" on the page.
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<StructureSection load='3hbr' size='340' side='right'caption='[[3hbr]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hbr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HBR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
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{{STRUCTURE_3hbr| PDB=3hbr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hbr OCA], [https://pdbe.org/3hbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hbr RCSB], [https://www.ebi.ac.uk/pdbsum/3hbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hbr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6XEC0_KLEPN Q6XEC0_KLEPN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hb/3hbr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hbr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Carbapenem-hydrolyzing class D beta-lactamases (CHDLs) are enzymes found in important Gram-negative pathogens (mainly Acinetobacter baumannii and Enterobacteriaceae) that confer resistance to beta-lactam antibiotics, and notably carbapenems. The crystal structure of the OXA-48 carbapenemase was determined at pH 7.5 and at a resolution of 1.9 A. Surprisingly, and by contrast with OXA-24, the only other CHDL of known crystal structure, the structure of OXA-48 was similar to OXA-10, an enzyme devoid of carbapenemase activity, indicating that the hydrolysis of these compounds could depend on subtle changes in the active site region. Moreover, the active site groove of OXA-48 was different from that of OXA-24 in shape, dimensions, and charge distribution. Molecular dynamics pointed to the functional relevance of residues located in or close to the beta5-beta6 loop and allowed us to propose a mechanism for carbapenem hydrolysis by OXA-48.
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===Crystal structure of OXA-48 beta-lactamase===
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Crystal structure of the OXA-48 beta-lactamase reveals mechanistic diversity among class D carbapenemases.,Docquier JD, Calderone V, De Luca F, Benvenuti M, Giuliani F, Bellucci L, Tafi A, Nordmann P, Botta M, Rossolini GM, Mangani S Chem Biol. 2009 May 29;16(5):540-7. PMID:19477418<ref>PMID:19477418</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3hbr" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19477418}}, adds the Publication Abstract to the page
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*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19477418 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19477418}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3hbr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HBR OCA].
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==Reference==
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<ref group="xtra">PMID:19477418</ref><ref group="xtra">PMID:11724923</ref><references group="xtra"/>
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[[Category: Beta-lactamase]]
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[[Category: Klebsiella pneumoniae]]
[[Category: Klebsiella pneumoniae]]
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[[Category: Benvenuti, M.]]
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[[Category: Large Structures]]
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[[Category: Calderone, V.]]
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[[Category: Benvenuti M]]
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[[Category: Docquier, J D.]]
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[[Category: Calderone V]]
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[[Category: Mangani, S.]]
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[[Category: Docquier JD]]
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[[Category: Rossolini, G M.]]
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[[Category: Mangani S]]
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[[Category: Antibiotic]]
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[[Category: Rossolini GM]]
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[[Category: Class d beta-lactamase]]
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[[Category: Dimer]]
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[[Category: Hydrolase]]
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[[Category: Oxa-48]]
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Current revision

Crystal structure of OXA-48 beta-lactamase

PDB ID 3hbr

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