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1t0o

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[[Image:1t0o.png|left|200px]]
 
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==The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose==
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The line below this paragraph, containing "STRUCTURE_1t0o", creates the "Structure Box" on the page.
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<StructureSection load='1t0o' size='340' side='right'caption='[[1t0o]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1t0o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T0O FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_1t0o| PDB=1t0o | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0o OCA], [https://pdbe.org/1t0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t0o RCSB], [https://www.ebi.ac.uk/pdbsum/1t0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t0o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q92456_HYPJE Q92456_HYPJE] Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.[ARBA:ARBA00003969]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t0/1t0o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t0o ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structures of alpha-galactosidase from the mesophilic fungus Trichoderma reesei and its complex with the competitive inhibitor, beta-d-galactose, have been determined at 1.54 A and 2.0 A resolution, respectively. The alpha-galactosidase structure was solved by the quick cryo-soaking method using a single Cs derivative. The refined crystallographic model of the alpha-galactosidase consists of two domains, an N-terminal catalytic domain of the (beta/alpha)8 barrel topology and a C-terminal domain which is formed by an antiparallel beta-structure. The protein contains four N-glycosylation sites located in the catalytic domain. Some of the oligosaccharides were found to participate in inter-domain contacts. The galactose molecule binds to the active site pocket located in the center of the barrel of the catalytic domain. Analysis of the alpha-galactosidase- galactose complex reveals the residues of the active site and offers a structural basis for identification of the putative mechanism of the enzymatic reaction. The structure of the alpha-galactosidase closely resembles those of the glycoside hydrolase family 27. The conservation of two catalytic Asp residues, identified for this family, is consistent with a double-displacement reaction mechanism for the alpha-galactosidase. Modeling of possible substrates into the active site reveals specific hydrogen bonds and hydrophobic interactions that could explain peculiarities of the enzyme kinetics.
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===The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose===
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Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism.,Golubev AM, Nagem RA, Brandao Neto JR, Neustroev KN, Eneyskaya EV, Kulminskaya AA, Shabalin KA, Savel'ev AN, Polikarpov I J Mol Biol. 2004 May 28;339(2):413-22. PMID:15136043<ref>PMID:15136043</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1t0o" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15136043}}, adds the Publication Abstract to the page
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*[[Galactosidase 3D structures|Galactosidase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15136043 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15136043}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[1t0o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0O OCA].
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[[Category: Trichoderma reesei]]
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[[Category: Brandao Neto JR]]
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==Reference==
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[[Category: Eneyskaya EV]]
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<ref group="xtra">PMID:15136043</ref><references group="xtra"/>
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[[Category: Golubev AM]]
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[[Category: Alpha-galactosidase]]
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[[Category: Kulminskaya AA]]
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[[Category: Hypocrea jecorina]]
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[[Category: Nagem RAP]]
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[[Category: Eneyskaya, E V.]]
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[[Category: Neustroev KN]]
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[[Category: Golubev, A M.]]
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[[Category: Polikarpov I]]
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[[Category: Kulminskaya, A A.]]
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[[Category: Savel'ev AN]]
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[[Category: Nagem, R A.P.]]
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[[Category: Shabalin KA]]
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[[Category: Neto, J R.Brandao.]]
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[[Category: Neustroev, K N.]]
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[[Category: Polikarpov, I.]]
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[[Category: Shabalin, K A.]]
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[[Category: Ev, A N.Savel.]]
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Current revision

The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose

PDB ID 1t0o

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