2h95

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(New page: 200px<br /><applet load="2h95" size="350" color="white" frame="true" align="right" spinBox="true" caption="2h95" /> '''Structure of the Amantadine-Blocked Influenz...)
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[[Image:2h95.jpg|left|200px]]<br /><applet load="2h95" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2h95" />
 
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'''Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy'''<br />
 
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==Overview==
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==Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy==
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Amantadine is known to block the M2 proton channel of the Influenza A, virus. Here, we present a structure of the M2trans-membrane domain blocked, with amantadine, built using orientational constraints obtained from solid, state NMR PISEMA experiments.The data indicates a kink in the monomer, between two helical fragments having 19 degrees and 28 degrees tilt angles, with respect to the membrane normal. This monomer structure is then used, to construct a plausible model of the tetrameric amantadine-blocked M2, trans-membrane channel. The influence of amantadine binding through, comparative Cross Polarization Magic Angle Spinning (CPMAS) spectra was, alsoobserved. In addition, spectra are shown of the amantadine resistant, mutant, S31N, in the presence and absence of amantadine.
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<StructureSection load='2h95' size='340' side='right'caption='[[2h95]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2h95]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/USSR/90/1977(H1N1)) Influenza A virus (A/USSR/90/1977(H1N1))]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H95 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h95 OCA], [https://pdbe.org/2h95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h95 RCSB], [https://www.ebi.ac.uk/pdbsum/2h95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h95 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q3LZC0_9INFA Q3LZC0_9INFA]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Amantadine is known to block the M2 proton channel of the Influenza A virus. Here, we present a structure of the M2 trans-membrane domain blocked with amantadine, built using orientational constraints obtained from solid-state NMR polarization-inversion-spin-exchange-at-the-magic-angle experiments. The data indicates a kink in the monomer between two helical fragments having 20 degrees and 31 degrees tilt angles with respect to the membrane normal. This monomer structure is then used to construct a plausible model of the tetrameric amantadine-blocked M2 trans-membrane channel. The influence of amantadine binding through comparative cross polarization magic-angle spinning spectra was also observed. In addition, spectra are shown of the amantadine-resistant mutant, S31N, in the presence and absence of amantadine.
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==About this Structure==
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Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus.,Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA Biophys J. 2007 Jun 15;92(12):4335-43. Epub 2007 Mar 23. PMID:17384070<ref>PMID:17384070</ref>
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2H95 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H95 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Backbone Structure of the Amantadine-blocked Trans-membrane Domain M2 Proton Channel from Influenza A Virus., Hu J, Asbury TM, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Mar 23;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17384070 17384070]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2h95" style="background-color:#fffaf0;"></div>
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[[Category: Asbury, T.]]
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[[Category: Cross, T.A.]]
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[[Category: Hu, J.]]
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[[Category: alpha helix]]
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[[Category: protein-ligand]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:42:46 2008''
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==See Also==
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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*[[M2 protein|M2 protein]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Asbury T]]
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[[Category: Cross TA]]
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[[Category: Hu J]]

Current revision

Structure of the Amantadine-Blocked Influenza A M2 Proton Channel Trans-membrane Domain by Solid-state NMR spectroscopy

PDB ID 2h95

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