2y8f

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[[Image:2y8f.png|left|200px]]
 
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==Structure of the Ran-binding domain from human RanBP3 (wild type)==
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The line below this paragraph, containing "STRUCTURE_2y8f", creates the "Structure Box" on the page.
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<StructureSection load='2y8f' size='340' side='right'caption='[[2y8f]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2y8f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y8F FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_2y8f| PDB=2y8f | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8f OCA], [https://pdbe.org/2y8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y8f RCSB], [https://www.ebi.ac.uk/pdbsum/2y8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8f ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RANB3_HUMAN RANB3_HUMAN] Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.<ref>PMID:9637251</ref> <ref>PMID:11571268</ref> <ref>PMID:11425870</ref> <ref>PMID:11932251</ref> <ref>PMID:19289081</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Proteins bearing a leucine-rich nuclear export signal (NES) are exported from the nucleus by the transport factor CRM1, which forms a cooperative ternary complex with the NES-bearing cargo and with the small GTPase Ran. CRM1-mediated export is regulated by RanBP3, a Ran-interacting nuclear protein. Unlike the related proteins RanBP1 and RanBP2, which promote disassembly of the export complex in the cytosol, RanBP3 acts as a CRM1 cofactor, enhancing NES export by stabilizing the export complex in the nucleus. RanBP3 also alters the cargo selectivity of CRM1, promoting recognition of the NES of HIV-1 Rev and of other cargos while deterring recognition of the import adaptor protein Snurportin1. Here we report the crystal structure of the Ran-binding domain (RBD) from RanBP3 and compare it to RBD structures from RanBP1 and RanBP2 in complex with Ran and CRM1. Differences among these structures suggest why RanBP3 binds Ran with unusually low affinity, how RanBP3 modulates the cargo selectivity of CRM1, and why RanBP3 promotes assembly rather than disassembly of the export complex. The comparison of RBD structures thus provides an insight into the functional diversity of Ran-binding proteins.
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===STRUCTURE OF THE RAN-BINDING DOMAIN FROM HUMAN RANBP3 (WILD TYPE)===
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Insights into the Function of the CRM1 Cofactor RanBP3 from the Structure of Its Ran-Binding Domain.,Langer K, Dian C, Rybin V, Muller CW, Petosa C PLoS One. 2011 Feb 25;6(2):e17011. PMID:21364925<ref>PMID:21364925</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2y8f" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21364925 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21364925}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2y8f]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8F OCA].
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==Reference==
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<ref group="xtra">PMID:21364925</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Dian, C.]]
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[[Category: Large Structures]]
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[[Category: Langer, K.]]
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[[Category: Dian C]]
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[[Category: Muller, C W.]]
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[[Category: Langer K]]
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[[Category: Petosa, C.]]
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[[Category: Muller CW]]
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[[Category: Rybin, V.]]
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[[Category: Petosa C]]
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[[Category: Rybin V]]

Current revision

Structure of the Ran-binding domain from human RanBP3 (wild type)

PDB ID 2y8f

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