3o72

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[[Image:3o72.jpg|left|200px]]
 
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==Crystal structure of EfeB in complex with heme==
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The line below this paragraph, containing "STRUCTURE_3o72", creates the "Structure Box" on the page.
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<StructureSection load='3o72' size='340' side='right'caption='[[3o72]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3o72]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7_str._TW14359 Escherichia coli O157:H7 str. TW14359]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O72 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
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{{STRUCTURE_3o72| PDB=3o72 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o72 OCA], [https://pdbe.org/3o72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o72 RCSB], [https://www.ebi.ac.uk/pdbsum/3o72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o72 ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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EfeB/YcdB is a member of the dye-decolorizing peroxidase (DyP) protein family. Recent study has shown that this protein can extract iron from heme without breaking the tetrapyrrole ring. We report the crystal structure of EfeB from E. coli O157 bound to heme at 1.95 Aresolution. The EfeB monomer contains two domains. The heme molecule is located in a large hydrophobic pocket in the C-terminal domain. A long loop connecting the two domains extensively interacts with the heme, which is a distinctive structural feature of EfeB homologues. A large tunnel formed by this loop and the beta-sheet of C-terminal domain provides a potential cofactor/substrate binding site. Biochemical data show that the production of protoporphyrin IX (PPIX) is closely related to the peroxidation activity. The mutant D235N keeps nearly the same activity of guaiacol peroxidase as the wild type protein, while the corresponding mutation in the classic DyP protein family completely abolishes the peroxidation activity. These results suggest that EfeB is a unique member of the DyP protein family. In addition, dramatically enhanced fluorescence excitation and emission of EfeB-PPIX was observed, implying this protein may be used as a red color fluorescence marker.
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===Crystal structure of EfeB in complex with heme===
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Crystal structure and biochemical features of EfeB/YcdB from Escherichia coli O157: Asp235 plays divergent roles in different enzyme-catalyzed processes.,Liu X, Du Q, Wang Z, Zhu D, Huang Y, Li N, Wei T, Xu S, Gu L J Biol Chem. 2011 Feb 15. PMID:21324904<ref>PMID:21324904</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3o72" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21324904 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21324904}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli O157:H7 str. TW14359]]
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[[3o72]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O72 OCA].
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[[Category: Large Structures]]
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[[Category: Du Q]]
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==Reference==
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[[Category: Gu L]]
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<ref group="xtra">PMID:21324904</ref><references group="xtra"/>
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[[Category: Huang Y]]
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[[Category: Escherichia coli]]
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[[Category: Li N]]
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[[Category: Du, Q.]]
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[[Category: Liu X]]
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[[Category: Gu, L.]]
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[[Category: Wang Z]]
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[[Category: Huang, Y.]]
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[[Category: Xu S]]
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[[Category: Li, N.]]
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[[Category: Zhu D]]
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[[Category: Liu, X.]]
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[[Category: Wang, Z.]]
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[[Category: Xu, S.]]
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[[Category: Zhu, D.]]
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Current revision

Crystal structure of EfeB in complex with heme

PDB ID 3o72

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