2y35

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[[Image:2y35.jpg|left|200px]]
 
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==Crystal structure of Xrn1-substrate complex==
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The line below this paragraph, containing "STRUCTURE_2y35", creates the "Structure Box" on the page.
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<StructureSection load='2y35' size='340' side='right'caption='[[2y35]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2y35]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y35 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_2y35| PDB=2y35 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y35 OCA], [https://pdbe.org/2y35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y35 RCSB], [https://www.ebi.ac.uk/pdbsum/2y35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y35 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/E1JJR3_DROME E1JJR3_DROME]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Messenger RNA decay plays a central role in the regulation and surveillance of eukaryotic gene expression. The conserved multidomain exoribonuclease Xrn1 targets cytoplasmic RNA substrates marked by a 5' monophosphate for processive 5'-to-3' degradation by an unknown mechanism. Here, we report the crystal structure of an Xrn1-substrate complex. The single-stranded substrate is held in place by stacking of the 5'-terminal trinucleotide between aromatic side chains while a highly basic pocket specifically recognizes the 5' phosphate. Mutations of residues involved in binding the 5'-terminal nucleotide impair Xrn1 processivity. The substrate recognition mechanism allows Xrn1 to couple processive hydrolysis to duplex melting in RNA substrates with sufficiently long single-stranded 5' overhangs. The Xrn1-substrate complex structure thus rationalizes the exclusive specificity of Xrn1 for 5'-monophosphorylated substrates, ensuring fidelity of mRNA turnover, and posits a model for translocation-coupled unwinding of structured RNA substrates.
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===CRYSTAL STRUCTURE OF XRN1-SUBSTRATE COMPLEX===
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Coupled 5' nucleotide recognition and processivity in xrn1-mediated mRNA decay.,Jinek M, Coyle SM, Doudna JA Mol Cell. 2011 Mar 4;41(5):600-8. PMID:21362555<ref>PMID:21362555</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_21362555}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2y35" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21362555 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21362555}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2y35]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y35 OCA].
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==Reference==
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<ref group="xtra">PMID:21362555</ref><references group="xtra"/>
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[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Coyle, S M.]]
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[[Category: Large Structures]]
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[[Category: Doudna, J A.]]
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[[Category: Synthetic construct]]
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[[Category: Jinek, M.]]
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[[Category: Coyle SM]]
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[[Category: Doudna JA]]
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[[Category: Jinek M]]

Current revision

Crystal structure of Xrn1-substrate complex

PDB ID 2y35

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