2pkg

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[[Image:2pkg.gif|left|200px]]<br /><applet load="2pkg" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2pkg, resolution 3.30&Aring;" />
 
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'''Structure of a complex between the A subunit of protein phosphatase 2A and the small t antigen of SV40'''<br />
 
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==Overview==
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==Structure of a complex between the A subunit of protein phosphatase 2A and the small t antigen of SV40==
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The small t antigen (ST) of DNA tumor virus SV40 facilitates cellular, transformation by disrupting the functions of protein phosphatase 2A, (PP2A) through a poorly defined mechanism. The crystal structure of the, core domain of SV40 ST bound to the scaffolding subunit of human PP2A, reveals that the ST core domain has a novel zinc-binding fold and, interacts with the conserved ridge of HEAT repeats 3-6, which overlaps, with the binding site for the B' (also called PR61 or B56) regulatory, subunit. ST has a lower binding affinity than B' for the PP2A core enzyme., Consequently, ST does not efficiently displace B' from PP2A holoenzymes in, vitro. Notably, ST inhibits PP2A phosphatase activity through its, N-terminal J domain. These findings suggest that ST may function mainly by, inhibiting the phosphatase activity of the PP2A core enzyme, and to a, lesser extent by modulating assembly of the PP2A holoenzymes.
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<StructureSection load='2pkg' size='340' side='right'caption='[[2pkg]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2pkg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Macaca_mulatta_polyomavirus_1 Macaca mulatta polyomavirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PKG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pkg OCA], [https://pdbe.org/2pkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pkg RCSB], [https://www.ebi.ac.uk/pdbsum/2pkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pkg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/2AAA_HUMAN 2AAA_HUMAN] The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.<ref>PMID:16580887</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pk/2pkg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pkg ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2PKG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PKG OCA].
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*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structural and biochemical insights into the regulation of protein phosphatase 2A by small t antigen of SV40., Chen Y, Xu Y, Bao Q, Xing Y, Li Z, Lin Z, Stock JB, Jeffrey PD, Shi Y, Nat Struct Mol Biol. 2007 Jun;14(6):527-34. Epub 2007 May 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17529992 17529992]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Simian virus 40]]
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[[Category: Macaca mulatta polyomavirus 1]]
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[[Category: Jeffrey, P.D.]]
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[[Category: Jeffrey PD]]
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[[Category: Shi, Y.]]
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[[Category: Shi Y]]
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[[Category: ZN]]
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[[Category: protein phosphatase 2a]]
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[[Category: regulation]]
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[[Category: small t antigen]]
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[[Category: sv40]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:47:56 2008''
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Current revision

Structure of a complex between the A subunit of protein phosphatase 2A and the small t antigen of SV40

PDB ID 2pkg

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