2pfz

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(New page: 200px<br /><applet load="2pfz" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pfz, resolution 1.800&Aring;" /> '''Crystal structure o...)
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[[Image:2pfz.jpg|left|200px]]<br /><applet load="2pfz" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2pfz, resolution 1.800&Aring;" />
 
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'''Crystal structure of DctP6, a Bordetella pertussis extracytoplasmic solute receptor binding pyroglutamic acid'''<br />
 
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==Overview==
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==Crystal structure of DctP6, a Bordetella pertussis extracytoplasmic solute receptor binding pyroglutamic acid==
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Gram-negative bacteria have developed several different transport systems, for solute uptake. One of these, the tripartite ATP independent, periplasmic transport system (TRAP-T), makes use of an extracytoplasmic, solute receptor (ESR) which captures specific solutes with high affinity, and transfers them to their partner permease complex located in the, bacterial inner membrane. We hereby report the structures of DctP6 and, DctP7, two such ESRs from Bordetella pertussis. These two proteins display, a high degree of sequence and structural similarity and possess the "Venus, flytrap" fold characteristic of ESRs, comprising two globular alpha/beta, domains hinged together to form a ligand binding cleft. DctP6 and DctP7, both show a closed conformation due to the presence of one pyroglutamic, acid molecule bound by highly conserved residues in their respective, ligand binding sites. BLAST analyses have revealed that the DctP6 and, DctP7 residues involved in ligand binding are strictly present in a number, of predicted TRAP-T ESRs from other bacteria. In most cases, the genes, encoding these TRAP-T systems are located in the vicinity of a gene coding, for a pyroglutamic acid metabolising enzyme. Both the high degree of, conservation of these ligand binding residues and the genomic context of, these TRAP-T-coding operons in a number of bacterial species, suggest that, DctP6 and DctP7 constitute the prototypes of a novel TRAP-T DctP subfamily, involved in pyroglutamic acid transport.
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<StructureSection load='2pfz' size='340' side='right'caption='[[2pfz]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2pfz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_pertussis_Tohama_I Bordetella pertussis Tohama I]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PFZ FirstGlance]. <br>
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2PFZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis] with <scene name='pdbligand=PCA:'>PCA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFZ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfz OCA], [https://pdbe.org/2pfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pfz RCSB], [https://www.ebi.ac.uk/pdbsum/2pfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pfz ProSAT]</span></td></tr>
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Crystal Structures of two Bordetella pertussis Periplasmic Receptors Contribute to Defining a Novel Pyroglutamic Acid Binding DctP Subfamily., Rucktooa P, Antoine R, Herrou J, Huvent I, Locht C, Jacob-Dubuisson F, Villeret V, Bompard C, J Mol Biol. 2007 Jun 29;370(1):93-106. Epub 2007 Apr 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17499270 17499270]
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</table>
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[[Category: Bordetella pertussis]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q7VXB3_BORPE Q7VXB3_BORPE]
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[[Category: Rucktooa, P.]]
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== Evolutionary Conservation ==
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[[Category: PCA]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: extracytoplasmic solute receptor]]
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Check<jmol>
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[[Category: ligand binding]]
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<jmolCheckbox>
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[[Category: pyroglutamic acid]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pf/2pfz_consurf.spt"</scriptWhenChecked>
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[[Category: tripartite atp independent periplasmic transport]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:56:03 2008''
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pfz ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Bordetella pertussis Tohama I]]
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[[Category: Large Structures]]
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[[Category: Rucktooa P]]

Current revision

Crystal structure of DctP6, a Bordetella pertussis extracytoplasmic solute receptor binding pyroglutamic acid

PDB ID 2pfz

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