2xsb

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[[Image:2xsb.jpg|left|200px]]
 
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==OgOGA PUGNAc complex==
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The line below this paragraph, containing "STRUCTURE_2xsb", creates the "Structure Box" on the page.
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<StructureSection load='2xsb' size='340' side='right'caption='[[2xsb]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xsb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oceanicola_granulosus_HTCC2516 Oceanicola granulosus HTCC2516]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XSB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GDL:2-ACETAMIDO-2-DEOXY-D-GLUCONO-1,5-LACTONE'>GDL</scene></td></tr>
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{{STRUCTURE_2xsb| PDB=2xsb | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xsb OCA], [https://pdbe.org/2xsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xsb RCSB], [https://www.ebi.ac.uk/pdbsum/2xsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xsb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OGA_OCEGH OGA_OCEGH] Cleaves GlcNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrate (in vitro).<ref>PMID:20863279</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Modification of cellular proteins with O-GlcNAc (O-linked N-acetylglucosamine) competes with protein phosphorylation and regulates a plethora of cellular processes. O-GlcNAcylation is orchestrated by two opposing enzymes, O-GlcNAc transferase and OGA (O-GlcNAcase or beta-N-acetylglucosaminidase), which recognize their target proteins via as yet unidentified mechanisms. In the present study, we uncovered the first insights into the mechanism of substrate recognition by human OGA. The structure of a novel bacterial OGA orthologue reveals a putative substrate-binding groove, conserved in metazoan OGAs. Guided by this structure, conserved amino acids lining this groove in human OGA were mutated and the activity on three different substrate proteins [TAB1 (transforming growth factor-beta-activated protein kinase 1-binding protein 1), FoxO1 (forkhead box O1) and CREB (cAMP-response-element-binding protein)] was tested in an in vitro deglycosylation assay. The results provide the first evidence that human OGA may possess a substrate-recognition mechanism that involves interactions with O-GlcNAcylated proteins beyond the GlcNAc-binding site, with possible implications for differential regulation of cycling of O-GlcNAc on different proteins.
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===OGOGA PUGNAC COMPLEX===
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Human OGA binds substrates in a conserved peptide recognition groove.,Schimpl M, Schuttelkopf AW, Borodkin VS, van Aalten DM Biochem J. 2010 Oct 25;432(1):1-7. PMID:20863279<ref>PMID:20863279</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xsb" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20863279}}, adds the Publication Abstract to the page
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*[[O-GlcNAcase|O-GlcNAcase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20863279 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20863279}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[2xsb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oceanicola_granulosus Oceanicola granulosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSB OCA].
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[[Category: Oceanicola granulosus HTCC2516]]
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[[Category: Schuttelkopf AW]]
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==Reference==
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[[Category: Van Aalten DMF]]
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<ref group="xtra">PMID:20863279</ref><references group="xtra"/>
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[[Category: Beta-N-acetylhexosaminidase]]
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[[Category: Oceanicola granulosus]]
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[[Category: Aalten, D M.F Van.]]
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[[Category: Schuttelkopf, A W.]]
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Current revision

OgOGA PUGNAc complex

PDB ID 2xsb

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