2p4b

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[[Image:2p4b.gif|left|200px]]<br /><applet load="2p4b" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2p4b, resolution 2.40&Aring;" />
 
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'''Crystal structure of E.coli RseB'''<br />
 
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==Overview==
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==Crystal structure of E.coli RseB==
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The bacterial envelope stress response senses stress signals in the, extracytoplasmic compartment, and activates sigma(E)-dependent, transcription by degrading its antisigma factor RseA. RseB, a binding, partner of RseA, plays a pivotal role in regulating this response, but its, molecular mechanism is not understood. We therefore determined the crystal, structure of Escherichia coli RseB at a resolution of 2.4 A. RseB is, composed of two domains linked by a flexible linker and forms a loosely, packed dimer with two grooves on each side. This structural feature is, confirmed by small-angle scattering in solution. Analysis of the binding, of various RseA mutants to RseB allowed us to identify the major, RseB-binding motif in RseA. These data, coupled with analysis of, small-angle scattering of the RseA/RseB complex in solution, leads us to, propose that two RseAs bind to the grooves of the dimeric RseB by, conserved residues. The implications for modulating proteolytic cleavage, of RseA are discussed.
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<StructureSection load='2p4b' size='340' side='right'caption='[[2p4b]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2p4b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P4B FirstGlance]. <br>
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2P4B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=BOG:'>BOG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P4B OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p4b OCA], [https://pdbe.org/2p4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p4b RCSB], [https://www.ebi.ac.uk/pdbsum/2p4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p4b ProSAT]</span></td></tr>
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Crystal structure of RseB and a model of its binding mode to RseA., Kim DY, Jin KS, Kwon E, Ree M, Kim KK, Proc Natl Acad Sci U S A. 2007 May 22;104(21):8779-84. Epub 2007 May 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17496148 17496148]
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</table>
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[[Category: Escherichia coli]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/RSEB_ECOLI RSEB_ECOLI] Seems to modulate the activity of RpoE (sigma-E).
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[[Category: Kim, D.Y.]]
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== Evolutionary Conservation ==
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[[Category: Kim, K.K.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: BOG]]
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Check<jmol>
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[[Category: open and closed form]]
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p4/2p4b_consurf.spt"</scriptWhenChecked>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:59:24 2008''
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p4b ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Kim DY]]
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[[Category: Kim KK]]

Current revision

Crystal structure of E.coli RseB

PDB ID 2p4b

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