2p6t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2p6t" size="350" color="white" frame="true" align="right" spinBox="true" caption="2p6t, resolution 2.90&Aring;" /> '''CRYSTAL STRUCTURE OF...)
Current revision (05:25, 17 October 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2p6t.jpg|left|200px]]<br /><applet load="2p6t" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2p6t, resolution 2.90&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF TRANSCRIPTIONAL REGULATOR NMB0573 and L-LEUCINE COMPLEX FROM NEISSERIA MENINGITIDIS'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF TRANSCRIPTIONAL REGULATOR NMB0573 and L-LEUCINE COMPLEX FROM NEISSERIA MENINGITIDIS==
-
Neisseria meningitidis, a causative agent of bacterial meningitis, has a, relatively small repertoire of transcription factors, including NMB0573, (annotated AsnC), a member of the Lrp-AsnC family of regulators that are, widely expressed in both Bacteria and Archaea. In the present study we, show that NMB0573 binds to L-leucine and L-methionine and have solved the, structure of the protein with and without bound amino acids. This has, shown, for the first time that amino acid binding does not induce, significant conformational changes in the structure of an AsnC/Lrp, regulator though it does appear to stabilise the octameric assembly of the, protein. Transcriptional profiling of wild type and NMB0573 knock-out, strains of N. meningitidis has shown that NMB0573 is associated with an, adaptive response to nutrient poor conditions reflected in a reduction in, major surface protein expression. On the basis of its structure and the, transcriptional response, we propose that NMB0573 is a global regulator in, Neisseria controlling responses to nutrient availability through, indicators of general amino acid abundance; leucine and methionine.
+
<StructureSection load='2p6t' size='340' side='right'caption='[[2p6t]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2p6t]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_MC58 Neisseria meningitidis MC58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P6T FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6t OCA], [https://pdbe.org/2p6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p6t RCSB], [https://www.ebi.ac.uk/pdbsum/2p6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p6t ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q9K0L9_NEIMB Q9K0L9_NEIMB]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/2p6t_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p6t ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Neisseria meningitidis, a causative agent of bacterial meningitis, has a relatively small repertoire of transcription factors, including NMB0573 (annotated AsnC), a member of the Lrp-AsnC family of regulators that are widely expressed in both Bacteria and Archaea. In the present study we show that NMB0573 binds to l-leucine and l-methionine and have solved the structure of the protein with and without bound amino acids. This has shown, for the first time that amino acid binding does not induce significant conformational changes in the structure of an AsnC/Lrp regulator although it does appear to stabilize the octameric assembly of the protein. Transcriptional profiling of wild-type and NMB0573 knock-out strains of N. meningitidis has shown that NMB0573 is associated with an adaptive response to nutrient poor conditions reflected in a reduction in major surface protein expression. On the basis of its structure and the transcriptional response, we propose that NMB0573 is a global regulator in Neisseria controlling responses to nutrient availability through indicators of general amino acid abundance: leucine and methionine.
-
==About this Structure==
+
The structure and transcriptional analysis of a global regulator from Neisseria meningitidis.,Ren J, Sainsbury S, Combs SE, Capper RG, Jordan PW, Berrow NS, Stammers DK, Saunders NJ, Owens RJ J Biol Chem. 2007 May 11;282(19):14655-64. Epub 2007 Mar 20. PMID:17374605<ref>PMID:17374605</ref>
-
2P6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_meningitidis_serogroup_b Neisseria meningitidis serogroup b] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=LEU:'>LEU</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6T OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
The structure and transcriptional analysis of a global regulator from neisseria meningitidis., Ren J, Sainsbury S, Combs SE, Capper RG, Jordan PW, Berrow NS, Stammers DK, Saunders NJ, Owens RJ, J Biol Chem. 2007 Mar 20;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17374605 17374605]
+
</div>
-
[[Category: Neisseria meningitidis serogroup b]]
+
<div class="pdbe-citations 2p6t" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
== References ==
-
[[Category: OPPF, Oxford.Protein.Production.Facility.]]
+
<references/>
-
[[Category: Owens, R.J.]]
+
__TOC__
-
[[Category: Ren, J.]]
+
</StructureSection>
-
[[Category: Sainsbury, S.]]
+
[[Category: Large Structures]]
-
[[Category: CA]]
+
[[Category: Neisseria meningitidis MC58]]
-
[[Category: GOL]]
+
[[Category: Owens RJ]]
-
[[Category: LEU]]
+
[[Category: Ren J]]
-
[[Category: lrp/asnc-family]]
+
[[Category: Sainsbury S]]
-
[[Category: n. meninigitidis]]
+
-
[[Category: nmb0573]]
+
-
[[Category: oppf]]
+
-
[[Category: oxford protein production facility]]
+
-
[[Category: structural and functional analysis of n. meninigitidis transcriptional regulators]]
+
-
[[Category: structural genomics]]
+
-
[[Category: transcriptional regulator]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:05:24 2008''
+

Current revision

CRYSTAL STRUCTURE OF TRANSCRIPTIONAL REGULATOR NMB0573 and L-LEUCINE COMPLEX FROM NEISSERIA MENINGITIDIS

PDB ID 2p6t

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2p6t"
Personal tools