2o3m

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(New page: 200px<br /><applet load="2o3m" size="350" color="white" frame="true" align="right" spinBox="true" caption="2o3m" /> '''Monomeric G-DNA tetraplex from human C-kit p...)
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[[Image:2o3m.gif|left|200px]]<br /><applet load="2o3m" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2o3m" />
 
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'''Monomeric G-DNA tetraplex from human C-kit promoter'''<br />
 
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==Overview==
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==Monomeric G-DNA tetraplex from human C-kit promoter==
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The c-kit oncogene is an important target in the treatment of, gastrointestinal tumors. A potential approach to inhibition of the, expression of this gene involves selective stabilization of G-quadruplex, structures that may be induced to form in the c-kit promoter region. Here, we report on the structure of an unprecedented intramolecular G-quadruplex, formed by a G-rich sequence in the c-kit promoter in K+ solution. The, structure represents a new folding topology with several unique features., Most strikingly, an isolated guanine is involved in G-tetrad core, formation, despite the presence of four three-guanine tracts. There are, four loops: two single-residue double-chain-reversal loops, a two-residue, loop, and a five-residue stem-loop, which contain base-pairing alignments., This unique structural scaffold provides a highly specific platform for, the future design of ligands specifically targeted to the promoter DNA of, c-kit.
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<StructureSection load='2o3m' size='340' side='right'caption='[[2o3m]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2o3m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O3M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O3M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o3m OCA], [https://pdbe.org/2o3m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o3m RCSB], [https://www.ebi.ac.uk/pdbsum/2o3m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o3m ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The c-kit oncogene is an important target in the treatment of gastrointestinal tumors. A potential approach to inhibition of the expression of this gene involves selective stabilization of G-quadruplex structures that may be induced to form in the c-kit promoter region. Here we report on the structure of an unprecedented intramolecular G-quadruplex formed by a G-rich sequence in the c-kit promoter in K+ solution. The structure represents a new folding topology with several unique features. Most strikingly, an isolated guanine is involved in G-tetrad core formation, despite the presence of four three-guanine tracts. There are four loops: two single-residue double-chain-reversal loops, a two-residue loop, and a five-residue stem-loop, which contain base-pairing alignments. This unique structural scaffold provides a highly specific platform for the future design of ligands specifically targeted to the promoter DNA of c-kit.
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==About this Structure==
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Structure of an unprecedented G-quadruplex scaffold in the human c-kit promoter.,Phan AT, Kuryavyi V, Burge S, Neidle S, Patel DJ J Am Chem Soc. 2007 Apr 11;129(14):4386-92. Epub 2007 Mar 16. PMID:17362008<ref>PMID:17362008</ref>
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2O3M is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O3M OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of an unprecedented G-quadruplex scaffold in the human c-kit promoter., Phan AT, Kuryavyi V, Burge S, Neidle S, Patel DJ, J Am Chem Soc. 2007 Apr 11;129(14):4386-92. Epub 2007 Mar 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17362008 17362008]
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</div>
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<div class="pdbe-citations 2o3m" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Burge, S.]]
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[[Category: Burge S]]
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[[Category: Kuryavyi, V.V.]]
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[[Category: Kuryavyi VV]]
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[[Category: Neidle, S.]]
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[[Category: Neidle S]]
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[[Category: Patel, D.J.]]
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[[Category: Patel DJ]]
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[[Category: Phan, A.T.]]
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[[Category: Phan AT]]
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[[Category: 5-nucleotide snap-back loops;broken strand topolog]]
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[[Category: g-tetrad;parallel g-quadruplex; edgewise]]
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[[Category: single-residue cha reversal]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:12:06 2008''
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Current revision

Monomeric G-DNA tetraplex from human C-kit promoter

PDB ID 2o3m

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