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3oxo

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[[Image:3oxo.png|left|200px]]
 
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==Succinyl-CoA:3-ketoacid CoA transferase from pig heart covalently bound to CoA==
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The line below this paragraph, containing "STRUCTURE_3oxo", creates the "Structure Box" on the page.
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<StructureSection load='3oxo' size='340' side='right'caption='[[3oxo]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3oxo]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OXO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OXO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
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{{STRUCTURE_3oxo| PDB=3oxo | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oxo OCA], [https://pdbe.org/3oxo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oxo RCSB], [https://www.ebi.ac.uk/pdbsum/3oxo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oxo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SCOT1_PIG SCOT1_PIG] Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Catalysis by succinyl-CoA:3-oxoacid CoA transferase proceeds through a thioester intermediate in which CoA is covalently linked to the enzyme. To determine the conformation of the thioester intermediate, crystals of the pig enzyme were grown in the presence of the substrate acetoacetyl-CoA. X-ray diffraction data show the enzyme in both the free form and covalently bound to CoA via Glu305. In the complex, the protein adopts a conformation in which residues 267-275, 280-287, 357-373 and 398-477 have shifted towards Glu305, closing the enzyme around the thioester. Enzymes provide catalysis by stabilizing the transition state relative to complexes with substrates or products. In this case, the conformational change allows the enzyme to interact with parts of CoA distant from the reactive thiol while the thiol is covalently linked to the enzyme. The enzyme forms stabilizing interactions with both the nucleotide and pantoic acid portions of CoA, while the interactions with the amide groups of the pantetheine portion are poor. The results shed light on how the enzyme uses the binding energy for groups remote from the active center of CoA to destabilize atoms closer to the active centre, leading to acceleration of the reaction by the enzyme.
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===Succinyl-CoA:3-ketoacid CoA transferase from pig heart covalently bound to CoA===
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Catalytic Role of the Conformational Change in Succinyl-CoA:3-Oxoacid CoA Transferase on Binding CoA.,Fraser ME, Hayakawa K, Brown WD Biochemistry. 2010 Oct 26. PMID:20977214<ref>PMID:20977214</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3oxo" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20977214 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20977214}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3oxo]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OXO OCA].
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==Reference==
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<ref group="xtra">PMID:20977214</ref><references group="xtra"/>
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[[Category: 3-oxoacid CoA-transferase]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Fraser, M E.]]
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[[Category: Fraser ME]]

Current revision

Succinyl-CoA:3-ketoacid CoA transferase from pig heart covalently bound to CoA

PDB ID 3oxo

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