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2x1o

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[[Image:2x1o.png|left|200px]]
 
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==Gelsolin Nanobody==
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The line below this paragraph, containing "STRUCTURE_2x1o", creates the "Structure Box" on the page.
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<StructureSection load='2x1o' size='340' side='right'caption='[[2x1o]], [[Resolution|resolution]] 1.34&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x1o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X1O FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2x1q|2x1q]], [[2x1p|2x1p]]</div></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x1o OCA], [https://pdbe.org/2x1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x1o RCSB], [https://www.ebi.ac.uk/pdbsum/2x1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x1o ProSAT]</span></td></tr>
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{{STRUCTURE_2x1o| PDB=2x1o | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RNA interference has tremendously advanced our understanding of gene function but recent reports have exposed undesirable side-effects. Recombinant Camelid single-domain antibodies (VHHs) provide an attractive means for studying protein function without affecting gene expression. We raised VHHs against gelsolin (GsnVHHs), a multifunctional actin-binding protein that controls cellular actin organization and migration. GsnVHH-induced delocalization of gelsolin to mitochondria or the nucleus in mammalian cells reveals distinct subpopulations including free gelsolin and actin-bound gelsolin complexes. GsnVHH 13 specifically recognizes Ca(2+)-activated gelsolin (K (d) approximately 10 nM) while GsnVHH 11 binds gelsolin irrespective of Ca(2+) (K (d) approximately 5 nM) but completely blocks its interaction with G-actin. Both GsnVHHs trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cell migration without affecting F-actin severing/capping or actin nucleation activities by gelsolin. We conclude that VHHs represent a potent way of blocking structural proteins and that actin nucleation by gelsolin is more complex than previously anticipated.
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===GELSOLIN NANOBODY===
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A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction.,Van den Abbeele A, De Clercq S, De Ganck A, De Corte V, Van Loo B, Soror SH, Srinivasan V, Steyaert J, Vandekerckhove J, Gettemans J Cell Mol Life Sci. 2010 May;67(9):1519-35. Epub 2010 Feb 7. PMID:20140750<ref>PMID:20140750</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20140750}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2x1o" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20140750 is the PubMed ID number.
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{{ABSTRACT_PUBMED_20140750}}
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==About this Structure==
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[[2x1o]] is a 2 chain structure of [[Gelsolin]] with sequence from [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X1O OCA].
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==See Also==
==See Also==
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*[[Gelsolin]]
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*[[Gelsolin 3D structures|Gelsolin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:20140750</ref><references group="xtra"/>
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__TOC__
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[[Category: Lama glama]]
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</StructureSection>
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[[Category: Abbeele, A Van Den.]]
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[[Category: Camelus glama]]
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[[Category: Corte, V De.]]
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[[Category: Large Structures]]
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[[Category: Declercq, S.]]
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[[Category: Abbeele, A Van Den]]
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[[Category: Ganck, A De.]]
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[[Category: Corte, V De]]
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[[Category: Gettemans, J.]]
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[[Category: Declercq, S]]
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[[Category: Kerckhove, J Van De.]]
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[[Category: Ganck, A De]]
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[[Category: Loo, B Van.]]
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[[Category: Gettemans, J]]
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[[Category: Srinivasan, V.]]
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[[Category: Kerckhove, J Van De]]
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[[Category: Steyaert, J.]]
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[[Category: Loo, B Van]]
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[[Category: Srinivasan, V]]
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[[Category: Steyaert, J]]
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[[Category: Contractile protein]]

Current revision

Gelsolin Nanobody

PDB ID 2x1o

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