2xva

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:39, 20 December 2023) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2xva.png|left|200px]]
 
-
<!--
+
==Crystal structure of the tellurite detoxification protein TehB from E. coli in complex with sinefungin==
-
The line below this paragraph, containing "STRUCTURE_2xva", creates the "Structure Box" on the page.
+
<StructureSection load='2xva' size='340' side='right'caption='[[2xva]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2xva]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._MG1655 Escherichia coli str. K-12 substr. MG1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XVA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XVA FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SFG:SINEFUNGIN'>SFG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_2xva| PDB=2xva | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xva OCA], [https://pdbe.org/2xva PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xva RCSB], [https://www.ebi.ac.uk/pdbsum/2xva PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xva ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/TEHB_ECOLI TEHB_ECOLI] S-adenosyl-L-methionine dependent methyltransferase that catalyzes the methylation of tellurite and is responsible for tellurite resistance when present in high copy number. Can also methylate selenite and selenium dioxide. Is thus able to detoxify different chalcogens. Can not methylate arsenic compounds.<ref>PMID:11053398</ref> <ref>PMID:21244361</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The oxyanion derivatives of the chalcogens tellurium and selenium are toxic to living organisms even at very low levels. Bacteria have developed mechanisms to overcome their toxicity by methylating them. The structure of the TehB from Escherichia coli has been determined in the presence of the co-factor analogues S-adenosyl-homocysteine (SAH) and sinefungin (non-hydrolysable form of S-adenosyl-L-methionine) at 1.48 A and 1.9 A respectively. Interestingly, our kinetic data show that TehB does not discriminate between selenium or tellurite oxyanions, making it a very powerful detoxifying protein. Analysis of the active site has identified three conserved residues that are capable of binding and orientating the metals for nucleophilic attack, His176, Arg177 and Arg184. Mutagenesis studies revealed that the His176Ala and Arg184Ala mutants retained most of their activity, whereas the Arg177Ala mutant had 65 % of its activity abolished. Based on the structure and kinetic data we propose an SN2 nucleophilic attack reaction mechanism. These data provide the first molecular understanding on the detoxification of chalcogens by bacteria.
-
===CRYSTAL STRUCTURE OF THE TELLURITE DETOXIFICATION PROTEIN TEHB FROM E. COLI IN COMPLEX WITH SINEFUNGIN===
+
Structure and mechanism of the chalcogen detoxifying protein TehB from Escherichia coli.,Choudhury HG, Cameron AD, Iwata S, Beis K Biochem J. 2011 Jan 18. PMID:21244361<ref>PMID:21244361</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_21244361}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2xva" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 21244361 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_21244361}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Escherichia coli str. K-12 substr. MG1655]]
-
[[2xva]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XVA OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Beis K]]
-
==Reference==
+
[[Category: Cameron AD]]
-
<ref group="xtra">PMID:21244361</ref><references group="xtra"/>
+
[[Category: Choudhury HG]]
-
[[Category: Escherichia coli]]
+
[[Category: Iwata S]]
-
[[Category: Beis, K.]]
+
-
[[Category: Cameron, A D.]]
+
-
[[Category: Choudhury, H G.]]
+
-
[[Category: Iwata, S.]]
+

Current revision

Crystal structure of the tellurite detoxification protein TehB from E. coli in complex with sinefungin

PDB ID 2xva

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xva"
Personal tools