2e9x

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[[Image:2e9x.jpg|left|200px]]<br /><applet load="2e9x" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2e9x, resolution 2.30&Aring;" />
 
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'''The crystal structure of human GINS core complex'''<br />
 
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==Overview==
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==The crystal structure of human GINS core complex==
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The eukaryotic GINS complex is essential for the establishment of DNA, replication forks and replisome progression. We report the crystal, structure of the human GINS complex. The heterotetrameric complex adopts a, pseudo symmetrical layered structure comprising two heterodimers, creating, four subunit-subunit interfaces. The subunit structures of the, heterodimers consist of two alternating domains. The C-terminal domains of, the Sld5 and Psf1 subunits are connected by linker regions to the core, complex, and the C-terminal domain of Sld5 is important for core complex, assembly. In contrast, the C-terminal domain of Psf1 does not contribute, to the stability of the complex but is crucial for chromatin binding and, replication activity. These data suggest that the core complex ensures a, stable platform for the C-terminal domain of Psf1 to act as a key, interaction interface for other proteins in the replication-initiation, process.
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<StructureSection load='2e9x' size='340' side='right'caption='[[2e9x]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2e9x]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E9X FirstGlance]. <br>
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2E9X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E9X OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e9x OCA], [https://pdbe.org/2e9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e9x RCSB], [https://www.ebi.ac.uk/pdbsum/2e9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e9x ProSAT]</span></td></tr>
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Structure of the human GINS complex and its assembly and functional interface in replication initiation., Kamada K, Kubota Y, Arata T, Shindo Y, Hanaoka F, Nat Struct Mol Biol. 2007 May;14(5):388-96. Epub 2007 Apr 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17417653 17417653]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PSF1_HUMAN PSF1_HUMAN] The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS1 is essential for function.<ref>PMID:17417653</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e9/2e9x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e9x ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Hanaoka, F.]]
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[[Category: Hanaoka F]]
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[[Category: Kamada, K.]]
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[[Category: Kamada K]]
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[[Category: SO4]]
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[[Category: eukaryotic dna replication]]
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[[Category: gins complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:14:39 2008''
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Current revision

The crystal structure of human GINS core complex

PDB ID 2e9x

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