2e5l

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[[Image:2e5l.gif|left|200px]]<br /><applet load="2e5l" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2e5l, resolution 3.300&Aring;" />
 
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'''A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction'''<br />
 
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==Overview==
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==A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction==
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In the initiation phase of bacterial translation, the 30S ribosomal, subunit captures mRNA in preparation for binding with initiator tRNA. The, purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of, the mRNA, anchors the 30S subunit near the start codon, via base pairing, with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we, present the 3.3 A crystal structure of the Thermus thermophilus 30S, subunit bound with an mRNA mimic. The duplex formed by the SD and aSD, sequences is snugly docked in a "chamber" between the head and platform, domains, demonstrating how the 30S subunit captures and stabilizes the, otherwise labile SD helix. This location of the SD helix is suitable for, the placement of the start codon AUG in the immediate vicinity of the mRNA, channel, in agreement with reported crosslinks between the second position, of the start codon and G1530 of 16S rRNA.
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<StructureSection load='2e5l' size='340' side='right'caption='[[2e5l]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2e5l]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E5L FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e5l OCA], [https://pdbe.org/2e5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e5l RCSB], [https://www.ebi.ac.uk/pdbsum/2e5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e5l ProSAT], [https://www.topsan.org/Proteins/RSGI/2e5l TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e5/2e5l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e5l ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2E5L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5L OCA].
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction., Kaminishi T, Wilson DN, Takemoto C, Harms JM, Kawazoe M, Schluenzen F, Hanawa-Suetsugu K, Shirouzu M, Fucini P, Yokoyama S, Structure. 2007 Mar;15(3):289-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17355865 17355865]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Synthetic construct]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Fucini, P.]]
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[[Category: Fucini P]]
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[[Category: Hanawa-Suetsugu, K.]]
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[[Category: Hanawa-Suetsugu K]]
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[[Category: Harms, J.M.]]
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[[Category: Harms JM]]
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[[Category: Kaminishi, T.]]
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[[Category: Kaminishi T]]
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[[Category: Kawazoe, M.]]
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[[Category: Kawazoe M]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Schluenzen F]]
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[[Category: Schluenzen, F.]]
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[[Category: Shirouzu M]]
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[[Category: Shirouzu, M.]]
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[[Category: Takemoto C]]
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[[Category: Takemoto, C.]]
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[[Category: Wilson DN]]
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[[Category: Wilson, D.N.]]
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[[Category: Yokoyama S]]
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[[Category: Yokoyama, S.]]
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[[Category: ZN]]
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[[Category: 30s ribosomal subunit]]
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[[Category: 5' untranslated region]]
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[[Category: mrna capture]]
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[[Category: national project on protein structural and functional analyses]]
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[[Category: nppsfa]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: shine-dalgarno interaction]]
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[[Category: structural genomics]]
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[[Category: translation initiation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:15:44 2008''
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Current revision

A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction

PDB ID 2e5l

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