2gzw

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[[Image:2gzw.jpg|left|200px]]<br /><applet load="2gzw" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gzw, resolution 2.21&Aring;" />
 
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'''Crystal structure of the E.coli CRP-cAMP complex'''<br />
 
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==Overview==
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==Crystal structure of the E.coli CRP-cAMP complex==
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The structure of a dimer of the Escherichia coli catabolite gene activator, protein has been refined at 2.5 A resolution to a crystallographic, R-factor of 20.7% starting with coordinates fitted to the map at 2.9 A, resolution. The two subunits are in different conformations and each, contains one bound molecule of the allosteric activator, cyclic AMP. The, amino-terminal domain is linked to the smaller carboxy-terminal domain by, a nine-residue hinge region that exists in different conformations in the, two subunits, giving rise to approximately a 30 degree rotation between, the positions of the small domains relative to the larger domains. The, amino-terminal domain contains an antiparallel beta-roll structure in, which the interstrand hydrogen bonding is well-determined. The beta-roll, can be described as a long antiparallel beta-ribbon that folds into a, right-handed supercoil and forms part of the cyclic AMP binding site. Each, cyclic AMP molecule is in an anti conformation and has ionic and hydrogen, bond interactions with both subunits.
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<StructureSection load='2gzw' size='340' side='right'caption='[[2gzw]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gzw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GZW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gzw OCA], [https://pdbe.org/2gzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gzw RCSB], [https://www.ebi.ac.uk/pdbsum/2gzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gzw ProSAT], [https://www.topsan.org/Proteins/RSGI/2gzw TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CRP_ECOLI CRP_ECOLI] This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.<ref>PMID:2982847</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/2gzw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gzw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2GZW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CMP:'>CMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GZW OCA].
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*[[Catabolite gene activator protein 3D structures|Catabolite gene activator protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of a complex of catabolite gene activator protein and cyclic AMP refined at 2.5 A resolution., Weber IT, Steitz TA, J Mol Biol. 1987 Nov 20;198(2):311-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2828639 2828639]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kumarevel, T.S.]]
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[[Category: Kumarevel TS]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Shinkai A]]
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[[Category: Shinkai, A.]]
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[[Category: Tanaka T]]
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[[Category: Tanaka, T.]]
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[[Category: Yokoyama S]]
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[[Category: Yokoyama, S.]]
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[[Category: CMP]]
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[[Category: camp binding protein]]
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[[Category: crp]]
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[[Category: national project on protein structural and functional analyses]]
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[[Category: nppsfa]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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[[Category: transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:15:55 2008''
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Current revision

Crystal structure of the E.coli CRP-cAMP complex

PDB ID 2gzw

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