2h09

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(New page: 200px<br /><applet load="2h09" size="350" color="white" frame="true" align="right" spinBox="true" caption="2h09, resolution 2.10&Aring;" /> '''Crystal structure of...)
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[[Image:2h09.jpg|left|200px]]<br /><applet load="2h09" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2h09, resolution 2.10&Aring;" />
 
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'''Crystal structure of diphtheria toxin repressor like protein from E. coli'''<br />
 
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==Overview==
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==Crystal structure of diphtheria toxin repressor like protein from E. coli==
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The virulent phenotype of the pathogenic bacterium Corynebacterium, diphtheriae is conferred by diphtheria toxin, whose expression is an, adaptive response to low concentrations of iron. The expression of the, toxin gene (tox) is regulated by the repressor DtxR, which is activated by, transition metal ions. X-ray crystal structures of DtxR with and without, (apo-form) its coordinated transition metal ion have established the, general architecture of the repressor, identified the location of the, metal-binding sites, and revealed a metal-ion-triggered subunit-subunit, 'caliper-like' conformational change. Here we report the three-dimensional, crystal structure of the complex between a biologically active, Ni(II)-bound DtxR(C102D) mutant, in which a cysteine is replaced by an, aspartate at residue 102, and a 33-base-pair DNA segment containing the, toxin operator toxO. This structure shows that DNA interacts with two, dimeric repressor proteins bound to opposite sides of the tox operator. We, propose that a metal-ion-induced helix-to-coil structural transition in, the amino-terminal region of the protein is partly responsible for the, unique mode of repressor activation by transition metal ions.
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<StructureSection load='2h09' size='340' side='right'caption='[[2h09]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2h09]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H09 FirstGlance]. <br>
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2H09 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H09 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h09 OCA], [https://pdbe.org/2h09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h09 RCSB], [https://www.ebi.ac.uk/pdbsum/2h09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h09 ProSAT], [https://www.topsan.org/Proteins/RSGI/2h09 TOPSAN]</span></td></tr>
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==Reference==
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</table>
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Structure of the metal-ion-activated diphtheria toxin repressor/tox operator complex., White A, Ding X, vanderSpek JC, Murphy JR, Ringe D, Nature. 1998 Jul 30;394(6692):502-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9697776 9697776]
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== Function ==
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[https://www.uniprot.org/uniprot/MNTR_ECOLI MNTR_ECOLI] In the presence of manganese, represses expression of mntH and mntS. Up-regulates expression of mntP.<ref>PMID:11466284</ref> <ref>PMID:21908668</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h0/2h09_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h09 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kumarevel, T.S.]]
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[[Category: Kumarevel TS]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Shinkai A]]
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[[Category: Shinkai, A.]]
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[[Category: Tanaka T]]
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[[Category: Tanaka, T.]]
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[[Category: Yokoyama S]]
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[[Category: Yokoyama, S.]]
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[[Category: diphtheria toxin]]
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[[Category: manganese transport]]
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[[Category: national project on protein structural and functional analyses]]
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[[Category: nppsfa]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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[[Category: transcription regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:15:58 2008''
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Current revision

Crystal structure of diphtheria toxin repressor like protein from E. coli

PDB ID 2h09

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