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Journal:JBIC:10
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| - | <StructureSection load=' | + | <StructureSection load='2z8q' size='450' side='right' scene='Journal:JBIC:10/Cv1/2' caption=''> |
=== Crystal structures of the all cysteinyl coordinated D14C variant of ''Pyrococcus furiosus'' ferredoxin: [4Fe-4S] <-> [3Fe-4S] cluster conversion === | === Crystal structures of the all cysteinyl coordinated D14C variant of ''Pyrococcus furiosus'' ferredoxin: [4Fe-4S] <-> [3Fe-4S] cluster conversion === | ||
| - | <big>Monika Nøhr Løvgreen, Maja Martic, Michael S. Windahl, Hans E. M. Christensen and Pernille Harris</big><ref>DOI</ref> | + | <big>Monika Nøhr Løvgreen, Maja Martic, Michael S. Windahl, Hans E. M. Christensen and Pernille Harris</big><ref>DOI 10.1007/s00775-011-0778-7</ref> |
<hr/> | <hr/> | ||
<b>Molecular Tour</b><br> | <b>Molecular Tour</b><br> | ||
| - | Structures of the all cysteinyl coordinated D14C variant of ferredoxin from the hyperthermophilic archaeon ''Pyrococcus furiosus'' have been determined for the [4Fe-4S] - and [3Fe-4S] forms. The [4Fe-4S] form diffracted to 1.7 Å and two different types of molecules were found in the crystal. They have different crystal packing and intramolecular disulfide bond conformation. The crystal packing reveals a beta-sheet interaction between A molecules in adjacent asymmetric units, while B molecules are packed as monomers in a less rigid position next to the A-A extended beta-sheet dimers. The intramolecular disulfide bond in the | + | Structures of the all cysteinyl coordinated D14C variant of ferredoxin from the hyperthermophilic archaeon ''Pyrococcus furiosus'' have been determined for the <scene name='Journal:JBIC:10/Cv1/5'>[4Fe-4S]</scene> <-> and <scene name='Journal:JBIC:10/Cv1/6'>[3Fe-4S]</scene> forms (<scene name='Journal:JBIC:10/Cv1/8'>click to enlarge</scene>). The [4Fe-4S] form diffracted to 1.7 Å and two different types of molecules were found in the crystal ([[2z8q]]). They have different crystal packing and intramolecular disulfide bond conformation. The crystal packing reveals a <scene name='Journal:JBIC:10/Cv/5'>beta-sheet interaction between A molecules</scene> (shown in <font color='blue'><b>blue</b></font> and <span style="color:green;background-color:black;font-weight:bold;">green</span>) in adjacent asymmetric units, while <scene name='Journal:JBIC:10/Cv/6'>B molecules are packed as monomers in a less rigid position next to the A-A extended beta-sheet dimers</scene> (shown in <font color='red'><b>red</b></font> and <span style="color:yellow;background-color:black;font-weight:bold;">yellow</span>). The <scene name='Journal:JBIC:10/Cv1/9'>intramolecular disulfide bond in the A molecules is in a double conformation</scene> while the intramolecular disulfide bond in the <scene name='Journal:JBIC:10/Cv1/10'>B molecules is in a single conformation</scene> (<scene name='Journal:JBIC:10/Cv1/11'>click to see morph</scene>, molecule A is shown in <font color='blue'><b>blue</b></font> and molecule B in <span style="color:green;background-color:black;font-weight:bold;">green</span>). |
| - | A molecules is in a double conformation while the intramolecular disulfide bond in the B molecules is in a single conformation. | + | |
| - | Two forms of D14C [3Fe-4S] ''Pyrococcus furiosus'' ferredoxin are obtained when purified at pH 8.0: a monomer and a dimer connected by an intermolecular disulfide bond. When purified at pH 5.8, only the monomer is obtained. The [3Fe-4S] form diffracted to 2.8 Å resolution and showed only the monomeric form, which resembles molecule A of D14C [4Fe-4S] ''Pyrococcus furiosus'' ferredoxin. | + | [[Image:Schem1.png|left|300px|thumb|pH dependent equilibrium of D14C [3Fe-4S] ''P. furiosus'' ferredoxin between protonated and deprotonated monomers and formation of a disulfide bonded dimer from deprotonated monomers. Fd is short for ferredoxin.]] |
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| + | Two forms of D14C [3Fe-4S] ''Pyrococcus furiosus'' ferredoxin are obtained when purified at pH 8.0: a monomer and a dimer connected by an intermolecular disulfide bond (see static image at the left). When purified at pH 5.8, only the monomer is obtained. The [3Fe-4S] form diffracted to 2.8 Å resolution and showed only the <scene name='Journal:JBIC:10/Cv1/13'>monomeric form, which resembles molecule A of D14C [4Fe-4S] Pyrococcus furiosus ferredoxin</scene>. Crystal packing in <scene name='Journal:JBIC:10/Cv2/7'>D14C [3Fe-4S] ferredoxin is as extended beta-sheet dimers of adjacent molecules</scene> (shown in <font color='red'><b>red</b></font> and <font color='orange'><b>orange</b></font>), which is the same as <scene name='Journal:JBIC:10/Cv2/9'>WT [3Fe-4S] P. furiosus ferredoxin</scene> ([[1sj1]], shown in <font color='blue'><b>blue</b></font> and <font color='cyan'><b>cyan</b></font>) even though the space groups are different (see also corresponding side views for <scene name='Journal:JBIC:10/Cv2/8'>D14C [3Fe-4S]</scene>) and <scene name='Journal:JBIC:10/Cv2/10'>WT [3Fe-4S]</scene>). | ||
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| + | '''PDB reference:''' Crystal structure of D14C [3Fe-4S] ''Pyrococcus furiosus'' ferredoxin, [[3pni]]. | ||
</StructureSection> | </StructureSection> | ||
Current revision
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![pH dependent equilibrium of D14C [3Fe-4S] P. furiosus ferredoxin between protonated and deprotonated monomers and formation of a disulfide bonded dimer from deprotonated monomers. Fd is short for ferredoxin.](/wiki/index.php/Image:Schem1.png)
- ↑ Lovgreen MN, Martic M, Windahl MS, Christensen HE, Harris P. Crystal structures of the all-cysteinyl-coordinated D14C variant of Pyrococcus furiosus ferredoxin: [4Fe-4S] <--> [3Fe-4S] cluster conversion. J Biol Inorg Chem. 2011 Apr 12. PMID:21484348 doi:10.1007/s00775-011-0778-7
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