3ojo

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[[Image:3ojo.png|left|200px]]
 
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==Derivative structure of the UDP-N-acetyl-mannosamine dehydrogenase Cap5O from S. aureus==
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The line below this paragraph, containing "STRUCTURE_3ojo", creates the "Structure Box" on the page.
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<StructureSection load='3ojo' size='340' side='right'caption='[[3ojo]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ojo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OJO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EU:EUROPIUM+ION'>EU</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PDC:PYRIDINE-2,6-DICARBOXYLIC+ACID'>PDC</scene></td></tr>
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{{STRUCTURE_3ojo| PDB=3ojo | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ojo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ojo OCA], [https://pdbe.org/3ojo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ojo RCSB], [https://www.ebi.ac.uk/pdbsum/3ojo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ojo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P95708_STAAU P95708_STAAU]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacterial UDP-sugar dehydrogenases are part of the biosynthesis pathway of extracellular polysaccharides. These compounds act as important virulence factors by protecting the cell from opsonophagocytosis and complement-mediated killing. In Staphylococcus aureus, the protein Cap5O catalyzes the oxidation of UDP-N-acetyl-mannosamine to UDP-N-acetyl-mannosaminuronic acid. Cap5O is crucial for the production of serotype 5 capsular polysaccharide that prevents the interaction of bacteria with both phagocytic and nonphagocytic eukaryotic cells. However, details of its catalytic mechanism remain unknown. We thus crystallized Cap5O and solved the first structure of an UDP-N-acetyl-mannosamine dehydrogenase. This study revealed that the catalytic cysteine makes a disulfide bond that has never been observed in other structurally characterized members of the NDP-sugar dehydrogenase family. Biochemical and mutagenesis experiments demonstrated that the formation of this disulfide bridge regulates the activity of Cap5O. We also identified two arginine residues essential for Cap5O activity. Previous data suggested that Cap5O is activated by tyrosine phosphorylation, so we characterized the phosphorylation site and examined the underlying regulatory mechanism.
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===Derivative structure of the UDP-N-acetyl-mannosamine dehydrogenase Cap5O from S. aureus===
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Structure analysis of the staphylococcus aureus UDP-N-acetyl-mannosamine dehydrogenase Cap5O involved in capsular polysaccharide biosynthesis.,Gruszczyk J, Fleurie A, Olivares-Illana V, Bechet E, Zanella-Cleon I, Morera S, Meyer P, Pompidor G, Kahn R, Grangeasse C, Nessler S J Biol Chem. 2011 Mar 23. PMID:21454499<ref>PMID:21454499</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ojo" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21454499 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21454499}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3ojo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJO OCA].
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==Reference==
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<ref group="xtra">PMID:21454499</ref><references group="xtra"/>
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Gruszczyk, J.]]
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[[Category: Gruszczyk J]]
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[[Category: Meyer, P.]]
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[[Category: Meyer P]]
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[[Category: Morera, S.]]
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[[Category: Morera S]]
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[[Category: Nessler, S.]]
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[[Category: Nessler S]]
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[[Category: Olivares-Illana, V.]]
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[[Category: Olivares-Illana V]]

Current revision

Derivative structure of the UDP-N-acetyl-mannosamine dehydrogenase Cap5O from S. aureus

PDB ID 3ojo

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