2otb

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[[Image:2otb.jpg|left|200px]]<br /><applet load="2otb" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2otb, resolution 1.790&Aring;" />
 
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'''Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state'''<br />
 
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==Overview==
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==Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state==
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Fluorescent protein (FP) variants that can be reversibly converted between, fluorescent and nonfluorescent states have proven to be a catalyst for, innovation in the field of fluorescence microscopy. However, the, structural basis of the process remains poorly understood. High-resolution, structures of a FP derived from Clavularia in both the fluorescent and the, light-induced nonfluorescent states reveal that the rapid and complete, loss of fluorescence observed upon illumination with 450-nm light results, from cis-trans isomerization of the chromophore. The photoinduced change, in configuration from the well ordered cis isomer to the highly nonplanar, and disordered trans isomer is accompanied by a dramatic rearrangement of, internal side chains. Taken together, the structures provide an, explanation for the loss of fluorescence upon illumination, the slow, light-independent recovery, and the rapid light-induced recovery of, fluorescence. The fundamental mechanism appears to be common to all of the, photoactivatable and reversibly photoswitchable FPs reported to date.
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<StructureSection load='2otb' size='340' side='right'caption='[[2otb]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2otb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clavularia_sp. Clavularia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OTB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2otb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2otb OCA], [https://pdbe.org/2otb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2otb RCSB], [https://www.ebi.ac.uk/pdbsum/2otb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2otb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFPL_CLASP GFPL_CLASP]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/2otb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2otb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Fluorescent protein (FP) variants that can be reversibly converted between fluorescent and nonfluorescent states have proven to be a catalyst for innovation in the field of fluorescence microscopy. However, the structural basis of the process remains poorly understood. High-resolution structures of a FP derived from Clavularia in both the fluorescent and the light-induced nonfluorescent states reveal that the rapid and complete loss of fluorescence observed upon illumination with 450-nm light results from cis-trans isomerization of the chromophore. The photoinduced change in configuration from the well ordered cis isomer to the highly nonplanar and disordered trans isomer is accompanied by a dramatic rearrangement of internal side chains. Taken together, the structures provide an explanation for the loss of fluorescence upon illumination, the slow light-independent recovery, and the rapid light-induced recovery of fluorescence. The fundamental mechanism appears to be common to all of the photoactivatable and reversibly photoswitchable FPs reported to date.
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==About this Structure==
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Structural basis for reversible photobleaching of a green fluorescent protein homologue.,Henderson JN, Ai HW, Campbell RE, Remington SJ Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6672-7. Epub 2007 Apr 9. PMID:17420458<ref>PMID:17420458</ref>
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2OTB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clavularia_sp. Clavularia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTB OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis for reversible photobleaching of a green fluorescent protein homologue., Henderson JN, Ai HW, Campbell RE, Remington SJ, Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6672-7. Epub 2007 Apr 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17420458 17420458]
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</div>
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[[Category: Clavularia sp.]]
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<div class="pdbe-citations 2otb" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Ai, H.]]
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[[Category: Campbell, R.E.]]
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[[Category: Henderson, J.N.]]
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[[Category: Remington, S.J.]]
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[[Category: beta can]]
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[[Category: fluorescent protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:19:37 2008''
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==See Also==
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Clavularia sp]]
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[[Category: Large Structures]]
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[[Category: Ai H]]
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[[Category: Campbell RE]]
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[[Category: Henderson JN]]
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[[Category: Remington SJ]]

Current revision

Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state

PDB ID 2otb

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