2hg2

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[[Image:2hg2.jpg|left|200px]]<br /><applet load="2hg2" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hg2, resolution 2.2&Aring;" />
 
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'''Structure of Lactaldehyde Dehydrogenase'''<br />
 
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==Overview==
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==Structure of Lactaldehyde Dehydrogenase==
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Aldehyde dehydrogenases catalyze the oxidation of aldehyde substrates to, the corresponding carboxylic acids. Lactaldehyde dehydrogenase from, Escherichia coli (aldA gene product, P25553) is an NAD(+)-dependent enzyme, implicated in the metabolism of l-fucose and l-rhamnose. During the, heterologous expression and purification of taxadiene synthase from the, Pacific yew, lactaldehyde dehydrogenase from E. coli was identified as a, minor (&lt;/=5%) side-product subsequent to its unexpected crystallization., Accordingly, we now report the serendipitous crystal structure, determination of unliganded lactaldehyde dehydrogenase from E. coli, determined by the technique of multiple isomorphous replacement using, anomalous scattering at 2.2 A resolution. Additionally, we report the, crystal structure of the ternary enzyme complex with products lactate and, NADH at 2.1 A resolution, and the crystal structure of the enzyme complex, with NADPH at 2.7 A resolution. The structure of the ternary complex, reveals that the nicotinamide ring of the cofactor is disordered between, two conformations: one with the ring positioned in the active site in the, so-called hydrolysis conformation, and another with the ring extended out, of the active site into the solvent region, designated the out, conformation. This represents the first crystal structure of an aldehyde, dehydrogenase-product complex. The active site pocket in which lactate, binds is more constricted than that of medium-chain dehydrogenases such as, the YdcW gene product of E. coli. The structure of the binary complex with, NADPH reveals the first view of the structural basis of specificity for, NADH: the negatively charged carboxylate group of E179 destabilizes the, binding of the 2'-phosphate group of NADPH sterically and, electrostatically, thereby accounting for the lack of enzyme activity with, this cofactor.
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<StructureSection load='2hg2' size='340' side='right'caption='[[2hg2]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hg2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HG2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hg2 OCA], [https://pdbe.org/2hg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hg2 RCSB], [https://www.ebi.ac.uk/pdbsum/2hg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hg2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALDA_ECOLI ALDA_ECOLI] Acts on lactaldehyde as well as other aldehydes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hg/2hg2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hg2 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2HG2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HG2 OCA].
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*[[Aldehyde dehydrogenase 3D structures|Aldehyde dehydrogenase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of lactaldehyde dehydrogenase from Escherichia coli and inferences regarding substrate and cofactor specificity., Di Costanzo L, Gomez GA, Christianson DW, J Mol Biol. 2007 Feb 16;366(2):481-93. Epub 2006 Nov 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17173928 17173928]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Christianson, D.W.]]
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[[Category: Christianson DW]]
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[[Category: Costanzo, L.Di.]]
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[[Category: Di Costanzo L]]
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[[Category: Gomez, G.A.]]
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[[Category: Gomez GA]]
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[[Category: SO4]]
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[[Category: dehydrogenase]]
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[[Category: nad dependent]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:20:33 2008''
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Current revision

Structure of Lactaldehyde Dehydrogenase

PDB ID 2hg2

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