2p0j

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(New page: 200px<br /><applet load="2p0j" size="350" color="white" frame="true" align="right" spinBox="true" caption="2p0j, resolution 2.100&Aring;" /> '''Structure of restri...)
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[[Image:2p0j.jpg|left|200px]]<br /><applet load="2p0j" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2p0j, resolution 2.100&Aring;" />
 
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'''Structure of restriction endonuclease BstYI bound to non-cognate DNA'''<br />
 
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==Overview==
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==Structure of restriction endonuclease BstYI bound to non-cognate DNA==
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DNA recognition by proteins is essential for specific expression of genes, in a living organism. En route to a target DNA site, a protein will often, sample noncognate DNA sites through nonspecific protein-DNA interactions, resulting in a variety of conformationally different binding states. We, present here the crystal structure of endonuclease BstYI bound to a, noncognate DNA. Surprisingly, the structure reveals the enzyme in a, "hemispecific" binding state on the pathway between nonspecific and, specific recognition. A single base pair change in the DNA abolishes, binding of only one monomer, with the second monomer bound specifically., We show that the enzyme binds essentially as a rigid body, and that one, end of the DNA is accommodated loosely in the binding cleft while the, other end is held tightly. Another intriguing feature of the structure is, Ser172, which has a dual role in establishing nonspecific and specific, contacts. Taken together, the structure provides a snapshot of an enzyme, in a "paused" intermediate state that may be part of a more general, mechanism of scanning DNA.
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<StructureSection load='2p0j' size='340' side='right'caption='[[2p0j]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2p0j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P0J FirstGlance]. <br>
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2P0J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P0J OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p0j OCA], [https://pdbe.org/2p0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p0j RCSB], [https://www.ebi.ac.uk/pdbsum/2p0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p0j ProSAT]</span></td></tr>
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==Reference==
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</table>
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BstYI Bound to Noncognate DNA Reveals a "Hemispecific" Complex: Implications for DNA Scanning., Townson SA, Samuelson JC, Bao Y, Xu SY, Aggarwal AK, Structure. 2007 Apr;15(4):449-59. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17437717 17437717]
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== Function ==
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[https://www.uniprot.org/uniprot/Q84AF2_GEOSE Q84AF2_GEOSE]
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__TOC__
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</StructureSection>
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Aggarwal, A.K.]]
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[[Category: Aggarwal AK]]
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[[Category: Bao, Y.]]
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[[Category: Bao Y]]
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[[Category: Samuelson, J.C.]]
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[[Category: Samuelson JC]]
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[[Category: Townson, S.A.]]
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[[Category: Townson SA]]
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[[Category: Xu, S.Y.]]
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[[Category: Xu SY]]
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[[Category: dna recognition]]
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[[Category: restriction endonuclease]]
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[[Category: scanning]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:28:51 2008''
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Current revision

Structure of restriction endonuclease BstYI bound to non-cognate DNA

PDB ID 2p0j

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