1li5

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[[Image:1li5.gif|left|200px]]<br /><applet load="1li5" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1li5, resolution 2.30&Aring;" />
 
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'''Crystal Structure of Cysteinyl-tRNA Synthetase'''<br />
 
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==Overview==
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==Crystal Structure of Cysteinyl-tRNA Synthetase==
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Cysteinyl-tRNA synthetase (CysRS) is highly specific for synthesis of, cysteinyl adenylate, yet does not possess the amino acid editing activity, characteristic of many other tRNA synthetases. To elucidate how CysRS is, able to distinguish cysteine from non-cognate amino acids, crystal, structures of the Escherichia coli enzyme were determined in apo and, cysteine-bound states. The structures reveal that the substrate cysteine, thiolate forms a single direct interaction with a zinc ion bound at the, base of the active site cleft, in a trigonal bipyramidal geometry together, with four highly conserved protein side chains. Cysteine binding induces, movement of the zinc ion towards substrate, as well as flipping of the, conserved Trp205 indole ring to pack on the thiol side chain. The, imidazole groups of five conserved histidines lie adjacent to the zinc, ion, forming a unique arrangement suggestive of functional significance., Thus, amino acid discrimination without editing arises most directly from, the favorable zinc-thiolate interaction, which is not possible for, non-cognate substrates. Additional selectivity may be generated during the, induced-fit conformational changes that help assemble the active site.
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<StructureSection load='1li5' size='340' side='right'caption='[[1li5]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1li5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LI5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1li5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1li5 OCA], [https://pdbe.org/1li5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1li5 RCSB], [https://www.ebi.ac.uk/pdbsum/1li5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1li5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYC_ECOLI SYC_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/li/1li5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1li5 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LI5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cysteine--tRNA_ligase Cysteine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.16 6.1.1.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LI5 OCA].
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural origins of amino acid selection without editing by cysteinyl-tRNA synthetase., Newberry KJ, Hou YM, Perona JJ, EMBO J. 2002 Jun 3;21(11):2778-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12032090 12032090]
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[[Category: Cysteine--tRNA ligase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hou, Y.M.]]
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[[Category: Hou Y-M]]
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[[Category: Newberry, K.J.]]
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[[Category: Newberry KJ]]
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[[Category: Perona, J.J.]]
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[[Category: Perona JJ]]
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[[Category: ZN]]
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[[Category: cysteine]]
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[[Category: e.coli]]
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[[Category: trna synthetase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:29:40 2008''
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Current revision

Crystal Structure of Cysteinyl-tRNA Synthetase

PDB ID 1li5

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