2oe5

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(New page: 200px<br /><applet load="2oe5" size="350" color="white" frame="true" align="right" spinBox="true" caption="2oe5, resolution 1.51&Aring;" /> '''1.5 A X-ray crystal ...)
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[[Image:2oe5.gif|left|200px]]<br /><applet load="2oe5" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2oe5, resolution 1.51&Aring;" />
 
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'''1.5 A X-ray crystal structure of Apramycin complex with RNA fragment GGCGUCGCUAGUACCG/GGUACUAAAAGUCGCCC containing the human ribosomal decoding A site: RNA construct with 3'-overhang'''<br />
 
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==Overview==
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==1.5 A X-ray crystal structure of Apramycin complex with RNA fragment GGCGUCGCUAGUACCG/GGUACUAAAAGUCGCCC containing the human ribosomal decoding A site: RNA construct with 3'-overhang==
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Aminoglycoside antibiotics bind specifically to the bacterial ribosomal, decoding-site RNA and thereby interfere with fidelity but not efficiency, of translation. Apramycin stands out among aminoglycosides for its, mechanism of action which is based on blocking translocation and its, ability to bind also to the eukaryotic decoding site despite differences, in key residues required for apramycin recognition by the bacterial, target. To elucidate molecular recognition of the eukaryotic decoding site, by apramycin we have determined the crystal structure of an, oligoribonucleotide containing the human sequence free and in complex with, the antibiotic at 1.5 A resolution. The drug binds in the deep groove of, the RNA which forms a continuously stacked helix comprising non-canonical, CA and GA base pairs and a bulged-out adenine. The binding mode of, apramycin at the human decoding-site RNA is distinct from aminoglycoside, recognition of the bacterial target, suggesting a molecular basis for the, actions of apramycin in eukaryotes and bacteria.
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<StructureSection load='2oe5' size='340' side='right'caption='[[2oe5]], [[Resolution|resolution]] 1.51&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2oe5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OE5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OE5 FirstGlance]. <br>
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2OE5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=AM2:'>AM2</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OE5 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.51&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AM2:APRAMYCIN'>AM2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oe5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oe5 OCA], [https://pdbe.org/2oe5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oe5 RCSB], [https://www.ebi.ac.uk/pdbsum/2oe5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oe5 ProSAT]</span></td></tr>
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Apramycin recognition by the human ribosomal decoding site., Hermann T, Tereshko V, Skripkin E, Patel DJ, Blood Cells Mol Dis. 2007 Jan 26;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17258916 17258916]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Hermann, T.]]
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</StructureSection>
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[[Category: Patel, D.J.]]
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[[Category: Large Structures]]
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[[Category: Skripkin, E.]]
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[[Category: Hermann T]]
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[[Category: Tereshko, V.]]
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[[Category: Patel DJ]]
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[[Category: AM2]]
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[[Category: Skripkin E]]
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[[Category: MG]]
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[[Category: Tereshko V]]
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[[Category: SO4]]
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[[Category: a site]]
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[[Category: aminoglycoside antibiotics]]
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[[Category: apramycin]]
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[[Category: homo sapiens]]
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[[Category: ribosomal decoding site]]
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[[Category: rna duplex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:31:59 2008''
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Current revision

1.5 A X-ray crystal structure of Apramycin complex with RNA fragment GGCGUCGCUAGUACCG/GGUACUAAAAGUCGCCC containing the human ribosomal decoding A site: RNA construct with 3'-overhang

PDB ID 2oe5

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