3p0g

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[[Image:3p0g.png|left|200px]]
 
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==Structure of a nanobody-stabilized active state of the beta2 adrenoceptor==
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The line below this paragraph, containing "STRUCTURE_3p0g", creates the "Structure Box" on the page.
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<StructureSection load='3p0g' size='340' side='right'caption='[[3p0g]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3p0g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. The April 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Nanobodies'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_4 10.2210/rcsb_pdb/mom_2011_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P0G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P0G:8-[(1R)-2-{[1,1-DIMETHYL-2-(2-METHYLPHENYL)ETHYL]AMINO}-1-HYDROXYETHYL]-5-HYDROXY-2H-1,4-BENZOXAZIN-3(4H)-ONE'>P0G</scene></td></tr>
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{{STRUCTURE_3p0g| PDB=3p0g | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p0g OCA], [https://pdbe.org/3p0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p0g RCSB], [https://www.ebi.ac.uk/pdbsum/3p0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p0g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ADRB2_HUMAN ADRB2_HUMAN] Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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G protein coupled receptors (GPCRs) exhibit a spectrum of functional behaviours in response to natural and synthetic ligands. Recent crystal structures provide insights into inactive states of several GPCRs. Efforts to obtain an agonist-bound active-state GPCR structure have proven difficult due to the inherent instability of this state in the absence of a G protein. We generated a camelid antibody fragment (nanobody) to the human beta(2) adrenergic receptor (beta(2)AR) that exhibits G protein-like behaviour, and obtained an agonist-bound, active-state crystal structure of the receptor-nanobody complex. Comparison with the inactive beta(2)AR structure reveals subtle changes in the binding pocket; however, these small changes are associated with an 11 A outward movement of the cytoplasmic end of transmembrane segment 6, and rearrangements of transmembrane segments 5 and 7 that are remarkably similar to those observed in opsin, an active form of rhodopsin. This structure provides insights into the process of agonist binding and activation.
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===Structure of a nanobody-stabilized active state of the beta2 adrenoceptor===
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Structure of a nanobody-stabilized active state of the beta(2) adrenoceptor.,Rasmussen SG, Choi HJ, Fung JJ, Pardon E, Casarosa P, Chae PS, Devree BT, Rosenbaum DM, Thian FS, Kobilka TS, Schnapp A, Konetzki I, Sunahara RK, Gellman SH, Pautsch A, Steyaert J, Weis WI, Kobilka BK Nature. 2011 Jan 13;469(7329):175-80. PMID:21228869<ref>PMID:21228869</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3p0g" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21228869}}, adds the Publication Abstract to the page
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*[[Adrenergic receptor 3D structures|Adrenergic receptor 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21228869 is the PubMed ID number.
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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{{ABSTRACT_PUBMED_21228869}}
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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== References ==
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==About this Structure==
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<references/>
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[[3p0g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_enterobacteria_phage_t4 Homo sapiens, enterobacteria phage t4] and [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. The April 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Nanobodies'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_4 10.2210/rcsb_pdb/mom_2011_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P0G OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Escherichia virus T4]]
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<ref group="xtra">PMID:21228869</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
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[[Category: Homo sapiens, enterobacteria phage t4]]
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[[Category: Lama glama]]
[[Category: Lama glama]]
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[[Category: Lysozyme]]
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[[Category: Large Structures]]
[[Category: Nanobodies]]
[[Category: Nanobodies]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Casarosa, P.]]
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[[Category: Casarosa P]]
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[[Category: Chae, P S.]]
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[[Category: Chae PS]]
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[[Category: Choi, H J.]]
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[[Category: Choi H-J]]
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[[Category: DeVree, B T.]]
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[[Category: DeVree BT]]
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[[Category: Fung, J J.]]
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[[Category: Fung JJ]]
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[[Category: Gellman, S H.]]
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[[Category: Gellman SH]]
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[[Category: Kobilka, B K.]]
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[[Category: Kobilka BK]]
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[[Category: Kobilka, T S.]]
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[[Category: Kobilka TS]]
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[[Category: Konetzki, I.]]
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[[Category: Konetzki I]]
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[[Category: Pardon, E.]]
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[[Category: Pardon E]]
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[[Category: Pautsch, A.]]
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[[Category: Pautsch A]]
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[[Category: Rasmussen, S G.F.]]
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[[Category: Rasmussen SGF]]
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[[Category: Rosenbaum, D M.]]
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[[Category: Rosenbaum DM]]
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[[Category: Schnapp, A.]]
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[[Category: Schnapp A]]
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[[Category: Steyaert, J.]]
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[[Category: Steyaert J]]
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[[Category: Sunahara, R K.]]
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[[Category: Sunahara RK]]
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[[Category: Thian, F S.]]
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[[Category: Thian FS]]
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[[Category: Weis, W I.]]
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[[Category: Weis WI]]

Current revision

Structure of a nanobody-stabilized active state of the beta2 adrenoceptor

PDB ID 3p0g

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