2wdw

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[[Image:2wdw.png|left|200px]]
 
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==The Native Crystal Structure of the Primary Hexose Oxidase (Dbv29) in Antibiotic A40926 Biosynthesis==
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The line below this paragraph, containing "STRUCTURE_2wdw", creates the "Structure Box" on the page.
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<StructureSection load='2wdw' size='340' side='right'caption='[[2wdw]], [[Resolution|resolution]] 3.21&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wdw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nonomuraea_gerenzanensis Nonomuraea gerenzanensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WDW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WDW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.21&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
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{{STRUCTURE_2wdw| PDB=2wdw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wdw OCA], [https://pdbe.org/2wdw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wdw RCSB], [https://www.ebi.ac.uk/pdbsum/2wdw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wdw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7WZ62_9ACTN Q7WZ62_9ACTN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/2wdw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wdw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In the search for new efficacious antibiotics, biosynthetic engineering offers attractive opportunities to introduce minor alterations to antibiotic structures that may overcome resistance. Dbv29, a flavin-containing oxidase, catalyzes the four-electron oxidation of a vancomycin-like glycopeptide to yield A40926. Structural and biochemical examination of Dbv29 now provides insights into residues that govern flavinylation and activity, protein conformation and reaction mechanism. In particular, the serendipitous discovery of a reaction intermediate in the crystal structure led us to identify an unexpected opportunity to intercept the normal enzyme mechanism at two different points to create new teicoplanin analogs. Using this method, we synthesized families of antibiotic analogs with amidated and aminated lipid chains, some of which showed marked potency and efficacy against multidrug resistant pathogens. This method offers a new strategy for the development of chemical diversity to combat antibacterial resistance.
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===THE NATIVE CRYSTAL STRUCTURE OF THE PRIMARY HEXOSE OXIDASE (DBV29) IN ANTIBIOTIC A40926 BIOSYNTHESIS===
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Interception of teicoplanin oxidation intermediates yields new antimicrobial scaffolds.,Liu YC, Li YS, Lyu SY, Hsu LJ, Chen YH, Huang YT, Chan HC, Huang CJ, Chen GH, Chou CC, Tsai MD, Li TL Nat Chem Biol. 2011 May;7(5):304-9. Epub 2011 Apr 10. PMID:21478878<ref>PMID:21478878</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_21478878}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2wdw" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21478878 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21478878}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[2wdw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nonomuraea_sp._atcc_39727 Nonomuraea sp. atcc 39727]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WDW OCA].
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[[Category: Nonomuraea gerenzanensis]]
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[[Category: Chan H-C]]
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==Reference==
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[[Category: Chen G-H]]
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<ref group="xtra">PMID:21478878</ref><references group="xtra"/>
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[[Category: Chen Y-H]]
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[[Category: Alcohol oxidase]]
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[[Category: Chou C-C]]
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[[Category: Nonomuraea sp. atcc 39727]]
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[[Category: Huang C-J]]
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[[Category: Chan, H C.]]
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[[Category: Huang Y-T]]
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[[Category: Chen, G H.]]
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[[Category: Li T-L]]
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[[Category: Chen, Y H.]]
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[[Category: Li Y-S]]
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[[Category: Chou, C C.]]
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[[Category: Liu Y-C]]
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[[Category: Huang, C J.]]
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[[Category: Lyu S-Y]]
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[[Category: Huang, Y T.]]
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[[Category: Tsai M-D]]
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[[Category: Li, T L.]]
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[[Category: Li, Y S.]]
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[[Category: Liu, Y C.]]
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[[Category: Lyu, S Y.]]
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[[Category: Tsai, M D.]]
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Current revision

The Native Crystal Structure of the Primary Hexose Oxidase (Dbv29) in Antibiotic A40926 Biosynthesis

PDB ID 2wdw

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