2y0g

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[[Image:2y0g.jpg|left|200px]]
 
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==X-ray structure of Enhanced Green Fluorescent Protein (EGFP)==
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The line below this paragraph, containing "STRUCTURE_2y0g", creates the "Structure Box" on the page.
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<StructureSection load='2y0g' size='340' side='right'caption='[[2y0g]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2y0g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. The June 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''GFP-like Proteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_6 10.2210/rcsb_pdb/mom_2014_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y0G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
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{{STRUCTURE_2y0g| PDB=2y0g | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y0g OCA], [https://pdbe.org/2y0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y0g RCSB], [https://www.ebi.ac.uk/pdbsum/2y0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y0g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Enhanced Green Fluorescent Protein (EGFP) is a variant of wild-type Green Fluorescent Protein from the jellyfish Aequorea victoria, whose mutations S65T and F64L increase brightness and folding efficiency. EGFP is extensively used in cell biology and biochemistry as a colocalization or expression reporter. Surprisingly, the structure of this very popular protein has not been determined yet. We report here its crystallographic structure at 1.5A resolution which shows significant differences in the vicinity of residue 64 and of the chromophore. In particular, two conformations are observed for the key residue glutamic acid 222, in apparent contradiction with the single fluorescence lifetime of the protein. We then show that X-ray induced decarboxylation of Glu222 during diffraction data collection results in the disruption of a hydrogen-bond network near the chromophore. Using single-crystal microspectrophotometry, we demonstrate that this correlates with a significant loss of the fluorescence properties. We thus propose a mechanism of bleaching of the protein at low temperature. Taken together, these two sets of results highlight the stabilizing role of Glu222 to the chromophore cavity of EGFP.
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===X-RAY STRUCTURE OF ENHANCED GREEN FLUORESCENT PROTEIN (EGFP)===
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Stabilizing role of glutamic acid 222 in the structure of Enhanced Green Fluorescent Protein.,Royant A, Noirclerc-Savoye M J Struct Biol. 2011 May;174(2):385-90. Epub 2011 Feb 16. PMID:21335090<ref>PMID:21335090</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2y0g" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21335090 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21335090}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2y0g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y0G OCA].
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==Reference==
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<ref group="xtra">PMID:21335090</ref><references group="xtra"/>
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[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
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[[Category: Noirclerc-Savoye, M.]]
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[[Category: GFP-like Proteins]]
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[[Category: Royant, A.]]
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Noirclerc-Savoye M]]
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[[Category: Royant A]]

Current revision

X-ray structure of Enhanced Green Fluorescent Protein (EGFP)

PDB ID 2y0g

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