1zo3

[[Image:1zo3.gif|left|200px]]<br /><applet load="1zo3" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1zo3" />

'''The P-site and P/E-site tRNA structures fitted to P/I site codon.'''<br />

==Overview==

The 70S ribosome and its complement of factors required for initiation of, translation in E. coli were purified separately and reassembled in vitro, with GDPNP, producing a stable initiation complex (IC) stalled after 70S, assembly. We have obtained a cryo-EM reconstruction of the IC showing, IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP, contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here, adopts a conformation radically different from that seen in the recent, crystal structure of IF2. The C-terminal domain of IF2 binds to the, single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into, a novel orientation at the P site. The GTP binding domain of IF2 binds to, the GTPase-associated center of the 50S subunit in a manner similar to, EF-G and EF-Tu. Additionally, we present evidence for the localization of, IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of, IF2 in the initiation complex.

==About this Structure==

1ZO3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO3 OCA].

 

The cryo-EM structure of a translation initiation complex from Escherichia coli., Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J, Cell. 2005 Jun 3;121(5):703-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15935757 15935757]

[[Category: Protein complex]]

[[Category: Allen, G.S.]]

[[Category: Ehrenberg, M.]]

[[Category: Frank, J.]]

[[Category: Gursky, R.]]

[[Category: Zavialov, A.]]