1zux

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(New page: 200px<br /><applet load="1zux" size="350" color="white" frame="true" align="right" spinBox="true" caption="1zux, resolution 1.850&Aring;" /> '''EosFP Fluorescent P...)
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[[Image:1zux.gif|left|200px]]<br /><applet load="1zux" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zux, resolution 1.850&Aring;" />
 
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'''EosFP Fluorescent Protein- Green Form'''<br />
 
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==Overview==
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==EosFP Fluorescent Protein- Green Form==
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Genetically encoded fusion constructs derived from fluorescent proteins, (FPs) can be designed to report on a multitude of events and signals in, cells, tissues, and entire organs without interfering with the complex, machinery of life. EosFP is a novel FP from the scleractinian coral, Lobophyllia hemprichii that switches its fluorescence emission from green, (516 nm) to red (581 nm) upon irradiation with approximately 400-nm light., This property enables localized tagging of proteins and thus provides a, valuable tool for tracking protein movements within live cells. Here, we, present the x-ray structures of the green and red forms of WT EosFP. They, reveal that formation of the red chromophore is associated with cleavage, of the peptide backbone, with surprisingly little change elsewhere in the, structure, and provide insights into the mechanism that generates this, interesting posttranslational polypeptide modification.
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<StructureSection load='1zux' size='340' side='right'caption='[[1zux]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zux]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZUX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zux OCA], [https://pdbe.org/1zux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zux RCSB], [https://www.ebi.ac.uk/pdbsum/1zux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zux ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q5S6Z9_LOBHE Q5S6Z9_LOBHE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zu/1zux_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zux ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Genetically encoded fusion constructs derived from fluorescent proteins (FPs) can be designed to report on a multitude of events and signals in cells, tissues, and entire organs without interfering with the complex machinery of life. EosFP is a novel FP from the scleractinian coral Lobophyllia hemprichii that switches its fluorescence emission from green (516 nm) to red (581 nm) upon irradiation with approximately 400-nm light. This property enables localized tagging of proteins and thus provides a valuable tool for tracking protein movements within live cells. Here, we present the x-ray structures of the green and red forms of WT EosFP. They reveal that formation of the red chromophore is associated with cleavage of the peptide backbone, with surprisingly little change elsewhere in the structure, and provide insights into the mechanism that generates this interesting posttranslational polypeptide modification.
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==About this Structure==
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Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP.,Nienhaus K, Nienhaus GU, Wiedenmann J, Nar H Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9156-9. Epub 2005 Jun 17. PMID:15964985<ref>PMID:15964985</ref>
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1ZUX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUX OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP., Nienhaus K, Nienhaus GU, Wiedenmann J, Nar H, Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9156-9. Epub 2005 Jun 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15964985 15964985]
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</div>
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[[Category: Lobophyllia hemprichii]]
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<div class="pdbe-citations 1zux" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Nar, H.]]
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[[Category: Nienhaus, G.U.]]
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[[Category: Nienhaus, K.]]
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[[Category: Wiedenmann, J.]]
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[[Category: beta-elimination]]
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[[Category: fluorescent protein]]
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[[Category: peptide bond cleavage]]
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[[Category: photoconversion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 17:43:25 2008''
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==See Also==
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Lobophyllia hemprichii]]
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[[Category: Nar H]]
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[[Category: Nienhaus GU]]
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[[Category: Nienhaus K]]
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[[Category: Wiedenmann J]]

Current revision

EosFP Fluorescent Protein- Green Form

PDB ID 1zux

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