1zyo

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(New page: 200px<br /><applet load="1zyo" size="350" color="white" frame="true" align="right" spinBox="true" caption="1zyo, resolution 2.40&Aring;" /> '''Crystal Structure of...)
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[[Image:1zyo.gif|left|200px]]<br /><applet load="1zyo" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zyo, resolution 2.40&Aring;" />
 
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'''Crystal Structure of the Serine Protease Domain of Sesbania Mosaic Virus polyprotein'''<br />
 
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==Overview==
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==Crystal Structure of the Serine Protease Domain of Sesbania Mosaic Virus polyprotein==
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Sesbania mosaic virus (SeMV) polyprotein is processed by its N-terminal, serine protease domain. The crystal structure of the protease domain was, determined to a resolution of 2.4 A using multiple isomorphous replacement, and anomalous scattering. The SeMV protease domain exhibited the, characteristic trypsin fold and was found to be closer to cellular serine, proteases than to other viral proteases. The residues of the S1-binding, pocket, H298, T279 and N308 were mutated to alanine in the, DeltaN70-Protease-VPg polyprotein, and the cis-cleavage activity was, examined. The H298A and T279A mutants were inactive, while the N308A, mutant was partially active, suggesting that the interactions of H298 and, T279 with P1-glutamate are crucial for the E-T/S cleavage. A region of, exposed aromatic amino acids, probably essential for interaction with VPg, was identified on the protease domain, and this interaction could play a, major role in modulating the function of the protease.
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<StructureSection load='1zyo' size='340' side='right'caption='[[1zyo]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zyo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sesbania_mosaic_virus Sesbania mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZYO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyo OCA], [https://pdbe.org/1zyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zyo RCSB], [https://www.ebi.ac.uk/pdbsum/1zyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zyo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9EB08_9VIRU Q9EB08_9VIRU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zy/1zyo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zyo ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Sesbania mosaic virus (SeMV) polyprotein is processed by its N-terminal serine protease domain. The crystal structure of the protease domain was determined to a resolution of 2.4 A using multiple isomorphous replacement and anomalous scattering. The SeMV protease domain exhibited the characteristic trypsin fold and was found to be closer to cellular serine proteases than to other viral proteases. The residues of the S1-binding pocket, H298, T279 and N308 were mutated to alanine in the DeltaN70-Protease-VPg polyprotein, and the cis-cleavage activity was examined. The H298A and T279A mutants were inactive, while the N308A mutant was partially active, suggesting that the interactions of H298 and T279 with P1-glutamate are crucial for the E-T/S cleavage. A region of exposed aromatic amino acids, probably essential for interaction with VPg, was identified on the protease domain, and this interaction could play a major role in modulating the function of the protease.
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==About this Structure==
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Crystal structure of the serine protease domain of Sesbania mosaic virus polyprotein and mutational analysis of residues forming the S1-binding pocket.,Gayathri P, Satheshkumar PS, Prasad K, Nair S, Savithri HS, Murthy MR Virology. 2006 Mar 15;346(2):440-51. Epub 2005 Dec 13. PMID:16356524<ref>PMID:16356524</ref>
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1ZYO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sesbania_mosaic_virus Sesbania mosaic virus] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glutamyl_endopeptidase Glutamyl endopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.19 3.4.21.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYO OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the serine protease domain of Sesbania mosaic virus polyprotein and mutational analysis of residues forming the S1-binding pocket., Gayathri P, Satheshkumar PS, Prasad K, Nair S, Savithri HS, Murthy MR, Virology. 2006 Mar 15;346(2):440-51. Epub 2005 Dec 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16356524 16356524]
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</div>
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[[Category: Glutamyl endopeptidase]]
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<div class="pdbe-citations 1zyo" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Sesbania mosaic virus]]
[[Category: Sesbania mosaic virus]]
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[[Category: Single protein]]
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[[Category: Gayathri P]]
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[[Category: Gayathri, P.]]
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[[Category: Murthy MRN]]
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[[Category: Murthy, M.R.N.]]
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[[Category: Prasad K]]
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[[Category: Prasad, K.]]
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[[Category: Satheshkumar PS]]
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[[Category: Satheshkumar, P.S.]]
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[[Category: Savithri HS]]
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[[Category: Savithri, H.S.]]
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[[Category: GOL]]
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[[Category: beta-barrel]]
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[[Category: glutamyl endopeptidase]]
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[[Category: viral serine protease of trypsin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 17:45:44 2008''
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Current revision

Crystal Structure of the Serine Protease Domain of Sesbania Mosaic Virus polyprotein

PDB ID 1zyo

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