This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1gxy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:25, 27 March 2024) (edit) (undo)
 
(22 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1gxy.gif|left|200px]]<br />
 
-
<applet load="1gxy" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1gxy, resolution 1.71&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF THE EUCARYOTIC MONO-ADP-RIBOSYLTRANSFERASE ART2.2; CRYSTAL FORM A (P21)'''<br />
 
-
==Overview==
+
==crystal structure of the eucaryotic mono-ADP-ribosyltransferase ART2.2; CRYSTAL FORM A (P21)==
-
The mammalian extracellular ADP-ribosyl transferases ART1 through ART5 are, sequence-related to each other. Among them ART2 is involved in immuno, regulation. The variant ART2.2 was expressed in the periplasm of, Escherichia coli and crystallized. Its structure was determined by X-ray, diffraction at 1.7A resolution in one crystal form and at slightly lower, resolutions in two others. The active center was indicated by a ligated, nicotinamide analogue, which also revealed a small induced-fit. The, centerpiece of the chainfold of ART2.2 agrees with those of all bacterial, ADP-ribosyl transferases. This correspondence and the nicotinamide, position were used to model the binding structure of the whole substrate, NAD(+) at ART2.2. Two of the bacterial enzymes are structurally more, closely ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12270706 (full description)]]
+
<StructureSection load='1gxy' size='340' side='right'caption='[[1gxy]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1gxy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GXY FirstGlance]. <br>
-
1GXY is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]] with GOL as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/NAD(P)(+)--protein-arginine_ADP-ribosyltransferase NAD(P)(+)--protein-arginine ADP-ribosyltransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.31 2.4.2.31]]. Structure known Active Site: GOA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GXY OCA]].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gxy OCA], [https://pdbe.org/1gxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gxy RCSB], [https://www.ebi.ac.uk/pdbsum/1gxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gxy ProSAT]</span></td></tr>
-
Structure of the ecto-ADP-ribosyl transferase ART2.2 from rat., Mueller-Dieckmann C, Ritter H, Haag F, Koch-Nolte F, Schulz GE, J Mol Biol. 2002 Sep 27;322(4):687-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12270706 12270706]
+
</table>
-
[[Category: NAD(P)(+)--protein-arginine ADP-ribosyltransferase]]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/NAR2B_RAT NAR2B_RAT] Has both NAD(+) glycohydrolase and ADP-ribosyltransferase activity (to a lesser extent).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gx/1gxy_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gxy ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
-
[[Category: Single protein]]
+
[[Category: Mueller-Dieckmann C]]
-
[[Category: Mueller-Dieckmann, C.]]
+
[[Category: Schulz GE]]
-
[[Category: Schulz, G.E.]]
+
-
[[Category: GOL]]
+
-
[[Category: adp-ribosyltransferase]]
+
-
[[Category: immuno-regulation]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:58:04 2007''
+

Current revision

crystal structure of the eucaryotic mono-ADP-ribosyltransferase ART2.2; CRYSTAL FORM A (P21)

PDB ID 1gxy

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gxy"
Personal tools