206d

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(New page: 200px<br /><applet load="206d" size="350" color="white" frame="true" align="right" spinBox="true" caption="206d, resolution 2.500&Aring;" /> '''BASE-PAIR OPENING A...)
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[[Image:206d.gif|left|200px]]<br /><applet load="206d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="206d, resolution 2.500&Aring;" />
 
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'''BASE-PAIR OPENING AND SPERMINE BINDING-B-DNA FEATURES DISPLAYED IN THE CRYSTAL STRUCTURE OF A GAL OPERON FRAGMENT: IMPLICATIONS FOR PROTEIN-DNA RECOGNITION'''<br />
 
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==Overview==
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==BASE-PAIR OPENING AND SPERMINE BINDING-B-DNA FEATURES DISPLAYED IN THE CRYSTAL STRUCTURE OF A GAL OPERON FRAGMENT: IMPLICATIONS FOR PROTEIN-DNA RECOGNITION==
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A sequence that is represented frequently in functionally important sites, involving protein-DNA interactions is GTG/CAC, suggesting that the trimer, may play a role in regulatory processes. The 2.5 A resolution structure of, d(CGGTGG)/d(CCACCG), a part of the interior operator (OI, nucleotides +44, to +49) of the gal operon, co-crystallized with spermine, is described, herein. The crystal packing arrangement in this structure is unprecedented, in a crystal of B-DNA, revealing a close packing of columns of stacked DNA, resembling a 5-stranded twisted wire cable. The final structure contains, one hexamer duplex, 17 water molecules and 1.5 spermine molecules per, crystallographic asymmetric unit. The hexamer exhibits base-pair opening, and shearing at T.A resulting in a novel non-Watson-Crick hydrogen-bonding, scheme between adenine and thymine in the GTG region. The ability of this, sequence to adopt unusual conformations in its GTG region may be a, critical factor conferring sequence selectivity on the binding of Gal, repressor. In addition, this is the first conclusive example of a crystal, structure of spermine with native B-DNA, providing insight into the, mechanics of polyamine-DNA binding, as well as possible explanations for, the biological action of spermine.
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<StructureSection load='206d' size='340' side='right'caption='[[206d]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[206d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=206D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=206D FirstGlance]. <br>
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206D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=206D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=206d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=206d OCA], [https://pdbe.org/206d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=206d RCSB], [https://www.ebi.ac.uk/pdbsum/206d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=206d ProSAT]</span></td></tr>
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Base-pair opening and spermine binding--B-DNA features displayed in the crystal structure of a gal operon fragment: implications for protein-DNA recognition., Tari LW, Secco AS, Nucleic Acids Res. 1995 Jun 11;23(11):2065-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7596838 7596838]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Secco, A.S.]]
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</StructureSection>
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[[Category: Tari, L.W.]]
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[[Category: Large Structures]]
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[[Category: SPM]]
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[[Category: Secco AS]]
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[[Category: b-dna]]
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[[Category: Tari LW]]
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[[Category: double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 17:46:59 2008''
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Current revision

BASE-PAIR OPENING AND SPERMINE BINDING-B-DNA FEATURES DISPLAYED IN THE CRYSTAL STRUCTURE OF A GAL OPERON FRAGMENT: IMPLICATIONS FOR PROTEIN-DNA RECOGNITION

PDB ID 206d

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