208d

[[Image:208d.gif|left|200px]]<br /><applet load="208d" size="350" color="white" frame="true" align="right" spinBox="true"

caption="208d, resolution 2.050&Aring;" />

'''HIGH-RESOLUTION STRUCTURE OF A DNA HELIX FORMING (C.G)*G BASE TRIPLETS'''<br />

==Overview==

Triple helices result from interaction between single- and double-stranded, nucleic acids. Their formation is a possible mechanism for recombination, of homologous gene sequences in nature and provides, inter alia, a basis, for artificial control of gene activity. Triple-helix motifs have been, extensively studied by a variety of techniques, but few high-resolution, structural data are available. The only triplet structures characterized, so far by X-ray diffraction were in protein-DNA complexes studied at about, 3 A resolution. We report here the X-ray analysis of a DNA nonamer, d(GCGAATTCG), to a resolution of 2.05 A, in which the extended crystal, structure contains (C.G)*G triplets as a fragment of triple helix. The, guanosine-containing chains are in a parallel orientation. This, arrangement is a necessary feature of models for homologous recombination, which results ultimately in replacement of one length of DNA by another of, similar sequence. The present-structure agrees with many published, predictions of triplex organization, and provides an accurate, representation of an element that allows sequence-specific association, between single- and double-stranded nucleic acids.

 

==About this Structure==

208D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=208D OCA].

 

High-resolution structure of a DNA helix forming (C.G)*G base triplets., Van Meervelt L, Vlieghe D, Dautant A, Gallois B, Precigoux G, Kennard O, Nature. 1995 Apr 20;374(6524):742-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7715732 7715732]

[[Category: Protein complex]]

[[Category: Dautant, A.]]

[[Category: Gallois, B.]]

[[Category: Kennard, O.]]

[[Category: Meervelt, L.Van.]]

[[Category: Precigoux, G.]]

[[Category: Vlieghe, D.]]