237d

[[Image:237d.jpg|left|200px]]<br /><applet load="237d" size="350" color="white" frame="true" align="right" spinBox="true"

caption="237d, resolution 2.500&Aring;" />

'''CRYSTAL STRUCTURE OF A DNA DECAMER SHOWING A NOVEL PSEUDO FOUR-WAY HELIX-HELIX JUNCTION'''<br />

==Overview==

The crystal structure of the decanucleotide d(CGCAATTGCG)2 has been solved, by a combination of molecular replacement and heavy-atom procedures and, has been refined to an R factor of 20.2% at 2.7 A. It is not a fully, base-paired duplex but has a central core of eight Watson-Crick base pairs, flanked by unpaired terminal guanosines and cytosines. These participate, in hydrogen-bonding arrangements with adjacent decamer duplexes in the, crystal lattice. The unpaired guanosines are bound in the G+C regions of, duplex minor grooves. The cytosines have relatively high mobility, even, though they are constrained to be in one region where they are involved in, base-paired triplets with G.C base pairs. The 5'-AATT sequence in the, duplex region has a narrow minor groove, providing further confirmation of, the sequence-dependent nature of groove width.

 

==About this Structure==

237D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=237D OCA].

 

==Reference==

Crystal structure of a DNA decamer showing a novel pseudo four-way helix-helix junction., Spink N, Nunn CM, Vojtechovsky J, Berman HM, Neidle S, Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10767-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7479880 7479880]

[[Category: Protein complex]]

[[Category: Berman, H.M.]]

[[Category: Neidle, S.]]

[[Category: Nunn, C.M.]]

[[Category: Spink, N.]]

[[Category: Vojtechovsky, J.]]

[[Category: b-dna]]

[[Category: double helix]]

[[Category: flipped-out bases]]

[[Category: intermolecular base triplet]]