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264d

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(New page: 200px<br /><applet load="264d" size="350" color="white" frame="true" align="right" spinBox="true" caption="264d, resolution 2.440&Aring;" /> '''THREE-DIMENSIONAL C...)
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[[Image:264d.jpg|left|200px]]<br /><applet load="264d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="264d, resolution 2.440&Aring;" />
 
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'''THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE A-TRACT DNA DODECAMER D(CGCAAATTTGCG) COMPLEXED WITH THE MINOR-GROOVE-BINDING DRUG HOECHST 33258'''<br />
 
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==Overview==
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==THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE A-TRACT DNA DODECAMER D(CGCAAATTTGCG) COMPLEXED WITH THE MINOR-GROOVE-BINDING DRUG HOECHST 33258==
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The molecular structure of the DNA A-tract dodecamer d(CGCAAATTTGCG), complexed with the drug Hoechst 33258 has been determined by X-ray, diffraction analysis. The Hoechst molecule binds in the DNA minor groove, covering the sequence AATTT of the central A-tract, with the piperazine, group close to one of the GC regions. The drug molecule makes two, three-centered hydrogen bonds from the nitrogen atoms of the benzimidazole, rings to the N3 and O2 atoms of the DNA bases. Although a high propeller, twist is observed in the A-tract, only one unsymmetrical three-centered, hydrogen bond is present in the DNA major groove. The structure is, compared with other minor-groove-binding drug complexes and the influence, of these drugs on DNA A-tracts is discussed.
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<StructureSection load='264d' size='340' side='right'caption='[[264d]], [[Resolution|resolution]] 2.44&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[264d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=264D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=264D FirstGlance]. <br>
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264D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=HT:'>HT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=264D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.44&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HT:2-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5-BI-BENZIMIDAZOLE'>HT</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=264d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=264d OCA], [https://pdbe.org/264d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=264d RCSB], [https://www.ebi.ac.uk/pdbsum/264d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=264d ProSAT]</span></td></tr>
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Three-dimensional crystal structure of the A-tract DNA dodecamer d(CGCAAATTTGCG) complexed with the minor-groove-binding drug Hoechst 33258., Vega MC, Garcia Saez I, Aymami J, Eritja R, Van der Marel GA, Van Boom JH, Rich A, Coll M, Eur J Biochem. 1994 Jun 15;222(3):721-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7517864 7517864]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Aymami, J.]]
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</StructureSection>
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[[Category: Boom, J.H.Van.]]
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[[Category: Large Structures]]
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[[Category: Coll, M.]]
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[[Category: Aymami J]]
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[[Category: Eritja, R.]]
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[[Category: Coll M]]
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[[Category: Garcia-Saez, I.]]
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[[Category: Eritja R]]
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[[Category: Marel, G.A.Van.Der.]]
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[[Category: Garcia-Saez I]]
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[[Category: Rich, A.]]
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[[Category: Rich A]]
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[[Category: Vega, M.C.]]
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[[Category: Van Boom JH]]
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[[Category: HT]]
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[[Category: Van Der Marel GA]]
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[[Category: b-dna]]
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[[Category: Vega MC]]
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[[Category: complexed with drug]]
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[[Category: double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 17:49:48 2008''
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Current revision

THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE A-TRACT DNA DODECAMER D(CGCAAATTTGCG) COMPLEXED WITH THE MINOR-GROOVE-BINDING DRUG HOECHST 33258

PDB ID 264d

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