2rh1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2rh1" [edit=sysop:move=sysop])
Current revision (08:38, 30 October 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2rh1.png|left|200px]]
 
-
<!--
+
==High resolution crystal structure of human B2-adrenergic G protein-coupled receptor.==
-
The line below this paragraph, containing "STRUCTURE_2rh1", creates the "Structure Box" on the page.
+
<StructureSection load='2rh1' size='340' side='right'caption='[[2rh1]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2rh1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The April 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Adrenergic Receptors'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_4 10.2210/rcsb_pdb/mom_2008_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RH1 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=ACM:ACETAMIDE'>ACM</scene>, <scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CAU:(2S)-1-(9H-CARBAZOL-4-YLOXY)-3-(ISOPROPYLAMINO)PROPAN-2-OL'>CAU</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_2rh1| PDB=2rh1 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh1 OCA], [https://pdbe.org/2rh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rh1 RCSB], [https://www.ebi.ac.uk/pdbsum/2rh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rh1 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ADRB2_HUMAN ADRB2_HUMAN] Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rh/2rh1_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rh1 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors constitute the largest family of eukaryotic signal transduction proteins that communicate across the membrane. We report the crystal structure of a human beta2-adrenergic receptor-T4 lysozyme fusion protein bound to the partial inverse agonist carazolol at 2.4 angstrom resolution. The structure provides a high-resolution view of a human G protein-coupled receptor bound to a diffusible ligand. Ligand-binding site accessibility is enabled by the second extracellular loop, which is held out of the binding cavity by a pair of closely spaced disulfide bridges and a short helical segment within the loop. Cholesterol, a necessary component for crystallization, mediates an intriguing parallel association of receptor molecules in the crystal lattice. Although the location of carazolol in the beta2-adrenergic receptor is very similar to that of retinal in rhodopsin, structural differences in the ligand-binding site and other regions highlight the challenges in using rhodopsin as a template model for this large receptor family.
-
===High resolution crystal structure of human B2-adrenergic G protein-coupled receptor.===
+
High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor.,Cherezov V, Rosenbaum DM, Hanson MA, Rasmussen SG, Thian FS, Kobilka TS, Choi HJ, Kuhn P, Weis WI, Kobilka BK, Stevens RC Science. 2007 Nov 23;318(5854):1258-65. Epub 2007 Oct 25. PMID:17962520<ref>PMID:17962520</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_17962520}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2rh1" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 17962520 is the PubMed ID number.
+
-
-->
+
-
{{ABSTRACT_PUBMED_17962520}}
+
-
 
+
-
==About this Structure==
+
-
[[2rh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_enterobacteria_phage_t4 Homo sapiens, enterobacteria phage t4]. The April 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Adrenergic Receptors'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_4 10.2210/rcsb_pdb/mom_2008_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH1 OCA].
+
==See Also==
==See Also==
-
*[[Beta-2 Adrenergic Receptor]]
+
*[[Adrenergic receptor|Adrenergic receptor]]
-
*[[Group:SMART:A Physical Model of the β2-Adrenergic Receptor]]
+
*[[Adrenergic receptor 3D structures|Adrenergic receptor 3D structures]]
-
*[[Group:SMART:Tangible Models of Cdc42 Interacting With Intersectin]]
+
*[[G protein-coupled receptor|G protein-coupled receptor]]
-
*[[User:Frieda S. Reichsman]]
+
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:017962520</ref><ref group="xtra">PMID:017962519</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
[[Category: Adrenergic Receptors]]
[[Category: Adrenergic Receptors]]
-
[[Category: Homo sapiens, enterobacteria phage t4]]
+
[[Category: Escherichia virus T4]]
 +
[[Category: Homo sapiens]]
 +
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
-
[[Category: ATCG3D, Accelerated Technologies Center for Gene to 3D Structure.]]
+
[[Category: Cherezov V]]
-
[[Category: Cherezov, V.]]
+
[[Category: Choi HJ]]
-
[[Category: Choi, H J.]]
+
[[Category: Hanson MA]]
-
[[Category: Hanson, M A.]]
+
[[Category: Kobilka BK]]
-
[[Category: Kobilka, B K.]]
+
[[Category: Kobilka TS]]
-
[[Category: Kobilka, T S.]]
+
[[Category: Kuhn P]]
-
[[Category: Kuhn, P.]]
+
[[Category: Rasmussen SGF]]
-
[[Category: Rasmussen, S G.F.]]
+
[[Category: Rosenbaum DM]]
-
[[Category: Rosenbaum, D M.]]
+
[[Category: Stevens RC]]
-
[[Category: Stevens, R C.]]
+
[[Category: Thian FS]]
-
[[Category: Thian, F S.]]
+
[[Category: Weis WI]]
-
[[Category: Weis, W I.]]
+
-
[[Category: 7tm]]
+
-
[[Category: Accelerated technologies center for gene to 3d structure]]
+
-
[[Category: Adrenergic]]
+
-
[[Category: Atcg3d]]
+
-
[[Category: Cholesterol]]
+
-
[[Category: Fusion]]
+
-
[[Category: Gpcr]]
+
-
[[Category: Lipidic]]
+
-
[[Category: Lipidic cubic phase]]
+
-
[[Category: Membrane protein]]
+
-
[[Category: Membrane protein / hydrolase complex]]
+
-
[[Category: Mesophase]]
+
-
[[Category: Protein structure initiative]]
+
-
[[Category: Psi-2]]
+
-
[[Category: Structural genomic]]
+

Current revision

High resolution crystal structure of human B2-adrenergic G protein-coupled receptor.

PDB ID 2rh1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2rh1"
Personal tools