Molecular Playground/Saposin C

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==This is a placeholder==
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<StructureSection load='2gtg' size='350' side='right' scene='Molecular_Playground/Saposin_C/Glycan_cys/1' caption='Saposin C domain of prosaposin'>
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This is a placeholder text to help you get started in
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One of the [[CBI Molecules]] being studied in the [http://www.umass.edu/cbi/ University of Massachusetts Amherst Chemistry-Biology Interface Program] at UMass Amherst and on display at the [http://www.molecularplayground.org/ Molecular Playground]
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placing a Jmol applet on your page. At any time, click
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Replace the PDB id (use lowercase!) after the STRUCTURE_ and after PDB= to load
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and display another structure.
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{{STRUCTURE_3cin | PDB=3cin | SCENE= }}
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One of the [[CBI Molecules]] being studied in the [http://www.umass.edu/cbi/ University of Massachusetts Amherst Chemistry-Biology Interface Program] at UMass Amherst and on display at the [http://www.molecularplayground.org/ Molecular Playground].
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== Saposin C domain of prosaposin ==
== Saposin C domain of prosaposin ==
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Prosaposin is a precursor of four cofactors, saposins A, B,C and D, which are required for hydrolysis of sphingolipids by lysosomal enzymes (1). Prosaposin contains multiple glycans and was found to be an obligate substrate for the UDP-glucose:glycoprotein glucosyl transferase 1 (UGT1), a folding sensor that resides in the ER and supports folding of secretory proteins(1). Deficiency of UGT1 causes defects in prosaposin maturation(1). Prosaposin is cleaved in the lysosome into the four saposins. The four saposins are members of the larger family of saposin –like proteins(2). The saposin motif is characterized by six <scene name='44/447911/Cys/4'>cysteines</scene> that form three intramolecular disulfide bonds(2). In this representation is shown the saposin C domain of prosaposin. Saposin C adopts a monomeric saposin fold with four <scene name='44/447911/Amphipatic/1'>amphipatic</scene> α-helices, where the third helix shows a highly localized kink (2). Shown in green is the <scene name='44/447911/Glycan/1'>side chain</scene> of an Asn residue that gets modified in the ER in the prosaposin precursor by addition of a glycan before it gets cleaved in the lysosome.
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==3D structures of saposin==
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<Structure load='2GTG' size='500' frame='true' align='right' caption='Insert caption here' scene='User:Abla_Tannous/Sandbox_1/Saposin_c/1'> The saposin C domain of prosaposin, an obligate substrate for the ER folding sensor UGT1
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<scene name='User:Abla_Tannous/Sandbox_1/Saposin_c/1'> The saposin C domain of prosaposin, an obligate substrate for the ER folding sensor UGT1</scene>
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Prosaposin is a precursor of four cofactors, saposins A, B,C and D, which are required for hydrolysis of sphingolipids by lysosomal enzymes (1). Prosaposin contains multiple glycans and was found to be an obligate substrate for the UDP-glucose:glycoprotein glucosyl transferase 1 (UGT1), a folding sensor that resides in the ER and supports folding of secretory proteins(1). Deficiency of UGT1 causes defects in prosaposin maturation(1). Prosaposin is cleaved in the lysosome into the four saposins. The four saposins are members of the larger family of saposin –like proteins(2). The saposin motif is characterized by six cysteines that form three intramolecular disulfide bonds(2). In this representation is shown the saposin C domain of prosaposin. Saposin C adopts a monomeric saposin fold with four amphipathic α-helices, where the third helix shows a highly localized kink (2).
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[[Saposin]]
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</StructureSection>
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==References==
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1- Bradley R. Pearse,Taku Tamura,Johan C. Sunryd,Gregory A. Grabowski, Randal J. Kaufman and Daniel N. Hebert. The role of UDP-Glc:glycoprotein glucosyltransferase 1 in the maturation of an obligate substrate prosaposin. Journal of Cell Biology. 189; 829-841 (2010)
1- Bradley R. Pearse,Taku Tamura,Johan C. Sunryd,Gregory A. Grabowski, Randal J. Kaufman and Daniel N. Hebert. The role of UDP-Glc:glycoprotein glucosyltransferase 1 in the maturation of an obligate substrate prosaposin. Journal of Cell Biology. 189; 829-841 (2010)
2- Victoria E. Ahn, Paul Leyko, Jean-René Alattia, Lu Chen, and Gilbert G. Privé. Crystal structures of saposin A and C. Protein Science 15;1849-1857 (2006)
2- Victoria E. Ahn, Paul Leyko, Jean-René Alattia, Lu Chen, and Gilbert G. Privé. Crystal structures of saposin A and C. Protein Science 15;1849-1857 (2006)

Current revision

Saposin C domain of prosaposin

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References

1- Bradley R. Pearse,Taku Tamura,Johan C. Sunryd,Gregory A. Grabowski, Randal J. Kaufman and Daniel N. Hebert. The role of UDP-Glc:glycoprotein glucosyltransferase 1 in the maturation of an obligate substrate prosaposin. Journal of Cell Biology. 189; 829-841 (2010)

2- Victoria E. Ahn, Paul Leyko, Jean-René Alattia, Lu Chen, and Gilbert G. Privé. Crystal structures of saposin A and C. Protein Science 15;1849-1857 (2006)

Proteopedia Page Contributors and Editors (what is this?)

Abla Tannous, Joel L. Sussman, Michal Harel, Alexander Berchansky

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