2ahq

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(New page: 200px<br /><applet load="2ahq" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ahq" /> '''Solution Structure of the C-terminal RpoN Do...)
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[[Image:2ahq.gif|left|200px]]<br /><applet load="2ahq" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ahq" />
 
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'''Solution Structure of the C-terminal RpoN Domain of Sigma-54 from Aquifex aeolicus'''<br />
 
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==Overview==
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==Solution Structure of the C-terminal RpoN Domain of Sigma-54 from Aquifex aeolicus==
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The "sigma" subunit of prokaryotic RNA polymerase allows gene-specific, transcription initiation. Two sigma families have been identified, sigma70, and sigma54, which use distinct mechanisms to initiate transcription and, share no detectable sequence homology. Although the sigma70-type factors, have been well characterized structurally by x-ray crystallography, no, high resolution structural information is available for the sigma54-type, factors. Here we present the NMR-derived structure of the C-terminal, domain of sigma54 from Aquifex aeolicus. This domain (Thr-323 to Gly-389), which contains the highly conserved RpoN box sequence, consists of a, poorly structured N-terminal tail followed by a three-helix bundle, which, is surprisingly similar to domains of the sigma70-type proteins. Residues, of the RpoN box, which have previously been shown to be critical for DNA, binding, form the second helix of an unpredicted helix-turn-helix motif., The homology of this structure with other DNA-binding proteins, combined, with previous biochemical data, suggests how the C-terminal domain of, sigma54 binds to DNA.
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<StructureSection load='2ahq' size='340' side='right'caption='[[2ahq]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ahq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AHQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ahq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahq OCA], [https://pdbe.org/2ahq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ahq RCSB], [https://www.ebi.ac.uk/pdbsum/2ahq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ahq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O66858_AQUAE O66858_AQUAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/2ahq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ahq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The "sigma" subunit of prokaryotic RNA polymerase allows gene-specific transcription initiation. Two sigma families have been identified, sigma70 and sigma54, which use distinct mechanisms to initiate transcription and share no detectable sequence homology. Although the sigma70-type factors have been well characterized structurally by x-ray crystallography, no high resolution structural information is available for the sigma54-type factors. Here we present the NMR-derived structure of the C-terminal domain of sigma54 from Aquifex aeolicus. This domain (Thr-323 to Gly-389), which contains the highly conserved RpoN box sequence, consists of a poorly structured N-terminal tail followed by a three-helix bundle, which is surprisingly similar to domains of the sigma70-type proteins. Residues of the RpoN box, which have previously been shown to be critical for DNA binding, form the second helix of an unpredicted helix-turn-helix motif. The homology of this structure with other DNA-binding proteins, combined with previous biochemical data, suggests how the C-terminal domain of sigma54 binds to DNA.
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==About this Structure==
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The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70.,Doucleff M, Malak LT, Pelton JG, Wemmer DE J Biol Chem. 2005 Dec 16;280(50):41530-6. Epub 2005 Oct 6. PMID:16210314<ref>PMID:16210314</ref>
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2AHQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHQ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70., Doucleff M, Malak LT, Pelton JG, Wemmer DE, J Biol Chem. 2005 Dec 16;280(50):41530-6. Epub 2005 Oct 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16210314 16210314]
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</div>
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[[Category: Aquifex aeolicus]]
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<div class="pdbe-citations 2ahq" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Doucleff, M.]]
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[[Category: Malak, L.T.]]
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[[Category: Pelton, J.G.]]
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[[Category: Wemmer, D.E.]]
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[[Category: rna polymerase]]
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[[Category: sigma factors]]
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[[Category: sigma-54]]
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[[Category: solution structure]]
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[[Category: transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:02:25 2008''
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==See Also==
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Aquifex aeolicus]]
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[[Category: Large Structures]]
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[[Category: Doucleff M]]
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[[Category: Malak LT]]
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[[Category: Pelton JG]]
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[[Category: Wemmer DE]]

Current revision

Solution Structure of the C-terminal RpoN Domain of Sigma-54 from Aquifex aeolicus

PDB ID 2ahq

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